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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
- ELISA [1]
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- Product number
- 500-P47-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDGF-BB Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PDGF-BB. Anti-Human PDGF-BB-specific antibody was purified by affinity chromatography employing an immobilized Human PDGF-BB matrix. Sandwich ELISA: To detect Human PDGF-BB by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PDGF-BB (500-P47Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human PDGF-BB. Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Regulation of cell proliferation and migration by TAK1 via transcriptional control of von Hippel-Lindau tumor suppressor.
Tan SH, Pal M, Tan MJ, Wong MH, Tam FU, Teo JW, Chong HC, Tan CK, Goh YY, Tang MB, Cheung PC, Tan NS
The Journal of biological chemistry 2009 Jul 3;284(27):18047-58
The Journal of biological chemistry 2009 Jul 3;284(27):18047-58
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect hPDGF-BB by Western Blot analysis PDGF-BB Polyclonal Antibody (Product # 500-P47-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect hPDGF-BB by Western Blot analysis PDGF-BB Polyclonal Antibody (Product # 500-P47-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human PDGF-BB by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of PDGF-BB Polyclonal Antibody (Product # 500-P47-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech PDGF-BB Polyclonal Antibody, Biotin (Product # 500-P47BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human PDGF-BB.
