PA5-19524

antibody from Invitrogen Antibodies

Targeting: PDGFB SIS, SSV

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA5-19524

Provider

Invitrogen Antibodies

Product name

PDGF-B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with rat and cow based on sequence homology. Store antibody at 4ºC for 1-2 weeks. For long-term storage, store at -20ºC.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The CCL2-CCR2 astrocyte-cancer cell axis in tumor extravasation at the brain.

Hajal C, Shin Y, Li L, Serrano JC, Jacks T, Kamm RD
Science advances 2021 Jun;7(26)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PDGF B protein using Product # PA5-19524, PDGF B primary antibody at a dilution of 1 µg/mL. Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot was performed using Anti-PDGF-B Polyclonal Antibody (Product # PA5-19524) and a 17 kDa band corresponding to Platelet-derived growth factor subunit B was observed across cell lines tested and was upregulated in cell lines upon PTI treatment. Also, the expression was found to be high in HUVEC when compared to SH-SH5Y. Whole cell extracts (30 µg lysate) of HMEC-1 (Lane 1), HUVEC (Lane 2), HUVEC treated with PTI (1X for 4 hrs) (Lane 3), SH-SY5Y (Lane 4), SH-SY5Y treated with PTI (1X for 4 hrs) (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).