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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-19524 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDGF-B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with rat and cow based on sequence homology.
- Concentration
- 0.8 mg/mL
Submitted references The CCL2-CCR2 astrocyte-cancer cell axis in tumor extravasation at the brain.
Hajal C, Shin Y, Li L, Serrano JC, Jacks T, Kamm RD
Science advances 2021 Jun;7(26)
Science advances 2021 Jun;7(26)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDGF B protein using Product # PA5-19524, PDGF B primary antibody at a dilution of 1 µg/mL. Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-PDGF-B Polyclonal Antibody (Product # PA5-19524) and a 17 kDa band corresponding to Platelet-derived growth factor subunit B was observed across cell lines tested and was upregulated in cell lines upon PTI treatment. Also, the expression was found to be high in HUVEC when compared to SH-SH5Y. Whole cell extracts (30 µg lysate) of HMEC-1 (Lane 1), HUVEC (Lane 2), HUVEC treated with PTI (1X for 4 hrs) (Lane 3), SH-SY5Y (Lane 4), SH-SY5Y treated with PTI (1X for 4 hrs) (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
