Explore
Validate
LearnMAB195-100
antibody from R&D Systems
Targeting: CCR6
BN-1, CD196, CKR-L3, CMKBR6, DCR2, DRY-6, GPR-CY4, GPR29, STRL22
Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [23]
- Comments [0]
- Validations
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB195-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCR6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CCR6. Stains human CCR6 transfectants but not the parental cell line in flow cytometry. Does not cross-react with transfectants expressing human CCR1, CCR2, CCR3, CCR4, CCR5, CCR7, CXCR1, CXCR2, CXCR3, CXCR4, or STRL33.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P51684- Isotype
- IgG
- Antibody clone number
- 53103
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The Cerebellum of Patients with Steatohepatitis Shows Lymphocyte Infiltration, Microglial Activation and Loss of Purkinje and Granular Neurons.
Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization.
Role of CCL20/CCR6 and the ERK signaling pathway in lung adenocarcinoma.
Circulating CCL20 as a New Biomarker of Abdominal Aortic Aneurysm.
Skewing toward Treg and Th2 responses is a characteristic feature of sustained remission in ANCA-positive granulomatosis with polyangiitis.
CCR6 expression in colon cancer is associated with advanced disease and supports epithelial-to-mesenchymal transition.
CCR6 is a prognostic marker for overall survival in patients with colorectal cancer, and its overexpression enhances metastasis in vivo.
Oxygen regulates human cytotrophoblast migration by controlling chemokine and receptor expression.
Increased plasmacytoid dendritic cells and RORγt-expressing immune effectors in cutaneous acute graft-versus-host disease.
Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency.
Identification of human NK17/NK1 cells.
CCL20/CCR6 feedback exaggerates epidermal growth factor receptor-dependent MUC5AC mucin production in human airway epithelial (NCI-H292) cells.
IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
Characterization of circulating dendritic cells in melanoma: role of CCR6 in plasmacytoid dendritic cell recruitment to the tumor.
Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis.
Characterisation of dendritic cell subsets in lung cancer micro-environments.
Paired synovium and lymph nodes from rheumatoid arthritis patients differ in dendritic cell and chemokine expression.
Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression.
Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire.
Balzano T, Forteza J, Molina P, Giner J, Monzó A, Sancho-Jiménez J, Urios A, Montoliu C, Felipo V
Scientific reports 2018 Feb 14;8(1):3004
Scientific reports 2018 Feb 14;8(1):3004
Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization.
Cecchinato V, Bernasconi E, Speck RF, Proietti M, Sauermann U, D'Agostino G, Danelon G, Rezzonico Jost T, Grassi F, Raeli L, Schöni-Affolter F, Stahl-Hennig C, Uguccioni M, Swiss HIV Cohort Study.
Journal of immunology (Baltimore, Md. : 1950) 2017 Jan 1;198(1):184-195
Journal of immunology (Baltimore, Md. : 1950) 2017 Jan 1;198(1):184-195
Role of CCL20/CCR6 and the ERK signaling pathway in lung adenocarcinoma.
Zhang XP, Hu ZJ, Meng AH, Duan GC, Zhao QT, Yang J
Oncology letters 2017 Dec;14(6):8183-8189
Oncology letters 2017 Dec;14(6):8183-8189
Circulating CCL20 as a New Biomarker of Abdominal Aortic Aneurysm.
Soto B, Gallastegi-Mozos T, Rodríguez C, Martínez-González J, Escudero JR, Vila L, Camacho M
Scientific reports 2017 Dec 11;7(1):17331
Scientific reports 2017 Dec 11;7(1):17331
Skewing toward Treg and Th2 responses is a characteristic feature of sustained remission in ANCA-positive granulomatosis with polyangiitis.
Szczeklik W, Jakieła B, Wawrzycka-Adamczyk K, Sanak M, Hubalewska-Mazgaj M, Padjas A, Surmiak M, Szczeklik K, Sznajd J, Musiał J
European journal of immunology 2017 Apr;47(4):724-733
European journal of immunology 2017 Apr;47(4):724-733
CCR6 expression in colon cancer is associated with advanced disease and supports epithelial-to-mesenchymal transition.
Kapur N, Mir H, Clark Iii CE, Krishnamurti U, Beech DJ, Lillard JW, Singh S
British journal of cancer 2016 Jun 14;114(12):1343-51
British journal of cancer 2016 Jun 14;114(12):1343-51
CCR6 is a prognostic marker for overall survival in patients with colorectal cancer, and its overexpression enhances metastasis in vivo.
Liu J, Ke F, Xu Z, Liu Z, Zhang L, Yan S, Wang Z, Wang H, Wang H
PloS one 2014;9(6):e101137
PloS one 2014;9(6):e101137
Oxygen regulates human cytotrophoblast migration by controlling chemokine and receptor expression.
Schanz A, Red-Horse K, Hess AP, Baston-Büst DM, Heiss C, Krüssel JS
Placenta 2014 Dec;35(12):1089-94
Placenta 2014 Dec;35(12):1089-94
Increased plasmacytoid dendritic cells and RORγt-expressing immune effectors in cutaneous acute graft-versus-host disease.
Malard F, Bossard C, Brissot E, Chevallier P, Guillaume T, Delaunay J, Mosnier JF, Moreau P, Grégoire M, Gaugler B, Mohty M
Journal of leukocyte biology 2013 Dec;94(6):1337-43
Journal of leukocyte biology 2013 Dec;94(6):1337-43
Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency.
Mazerolles F, Picard C, Kracker S, Fischer A, Durandy A
The Journal of allergy and clinical immunology 2013 Apr;131(4):1146-56, 1156.e1-5
The Journal of allergy and clinical immunology 2013 Apr;131(4):1146-56, 1156.e1-5
Identification of human NK17/NK1 cells.
Pandya AD, Al-Jaderi Z, Høglund RA, Holmøy T, Harbo HF, Norgauer J, Maghazachi AA
PloS one 2011;6(10):e26780
PloS one 2011;6(10):e26780
CCL20/CCR6 feedback exaggerates epidermal growth factor receptor-dependent MUC5AC mucin production in human airway epithelial (NCI-H292) cells.
Kim S, Lewis C, Nadel JA
Journal of immunology (Baltimore, Md. : 1950) 2011 Mar 15;186(6):3392-400
Journal of immunology (Baltimore, Md. : 1950) 2011 Mar 15;186(6):3392-400
IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Dec 15;17(24):7529-38
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Dec 15;17(24):7529-38
Characterization of circulating dendritic cells in melanoma: role of CCR6 in plasmacytoid dendritic cell recruitment to the tumor.
Charles J, Di Domizio J, Salameire D, Bendriss-Vermare N, Aspord C, Muhammad R, Lefebvre C, Plumas J, Leccia MT, Chaperot L
The Journal of investigative dermatology 2010 Jun;130(6):1646-56
The Journal of investigative dermatology 2010 Jun;130(6):1646-56
Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadière C, Mami-Chouaib F
Cancer research 2009 Aug 1;69(15):6249-55
Cancer research 2009 Aug 1;69(15):6249-55
Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3724-33
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3724-33
Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis.
Marchal-Sommé J, Uzunhan Y, Marchand-Adam S, Valeyre D, Soumelis V, Crestani B, Soler P
Journal of immunology (Baltimore, Md. : 1950) 2006 May 15;176(10):5735-9
Journal of immunology (Baltimore, Md. : 1950) 2006 May 15;176(10):5735-9
Characterisation of dendritic cell subsets in lung cancer micro-environments.
Bergeron A, El-Hage F, Kambouchner M, Lecossier D, Tazi A
The European respiratory journal 2006 Dec;28(6):1170-7
The European respiratory journal 2006 Dec;28(6):1170-7
Paired synovium and lymph nodes from rheumatoid arthritis patients differ in dendritic cell and chemokine expression.
Page G, Miossec P
The Journal of pathology 2004 Sep;204(1):28-38
The Journal of pathology 2004 Sep;204(1):28-38
Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression.
Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P
The American journal of pathology 2004 Feb;164(2):409-17
The American journal of pathology 2004 Feb;164(2):409-17
Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
Lim HW, Hillsamer P, Kim CH
The Journal of clinical investigation 2004 Dec;114(11):1640-9
The Journal of clinical investigation 2004 Dec;114(11):1640-9
Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
Blood 2004 Apr 1;103(7):2806-8
Blood 2004 Apr 1;103(7):2806-8
Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire.
Campbell JJ, Qin S, Unutmaz D, Soler D, Murphy KE, Hodge MR, Wu L, Butcher EC
Journal of immunology (Baltimore, Md. : 1950) 2001 Jun 1;166(11):6477-82
Journal of immunology (Baltimore, Md. : 1950) 2001 Jun 1;166(11):6477-82
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCR6 in Human Spleen. CCR6 was detected in immersion fixed paraffin-embedded sections of human spleen using 25 µg/mL Human CCR6 Monoclonal Antibody (Catalog # MAB195) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
