FAB590P-025

antibody from R&D Systems

Targeting: CCR6 BN-1, CD196, CKR-L3, CMKBR6, DCR2, DRY-6, GPR-CY4, GPR29, STRL22

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

FAB590P-025

Provider

R&D Systems

Product name

Human/Mouse CCR6 PE-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse CCR6-transfected rat RBL cells but not the parent cell line or cells transfected with mouse CCR5. Detects human CCR6 in flow cytometry.

Reactivity

Human, Mouse

Host

Rat

Antigen sequence

O54689


Isotype

IgG

Antibody clone number

140706

Vial size

25 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

References

Submitted references

DUSP10 constrains innate IL-33-mediated cytokine production in ST2^hi memory-type pathogenic Th2 cells.

Yamamoto T, Endo Y, Onodera A, Hirahara K, Asou HK, Nakajima T, Kanno T, Ouchi Y, Uematsu S, Nishimasu H, Nureki O, Tumes DJ, Shimojo N, Nakayama T
Nature communications 2018 Oct 12;9(1):4231

---

Infection of Female BWF1 Lupus Mice with Malaria Parasite Attenuates B Cell Autoreactivity by Modulating the CXCL12/CXCR4 Axis and Its Downstream Signals PI3K/AKT, NFκB and ERK.

Badr G, Sayed A, Abdel-Maksoud MA, Mohamed AO, El-Amir A, Abdel-Ghaffar FA, Al-Quraishy S, Mahmoud MH
PloS one 2015;10(4):e0125340

---

The chemokine receptor CCR6 facilitates the onset of mammary neoplasia in the MMTV-PyMT mouse model via recruitment of tumor-promoting macrophages.

Boyle ST, Faulkner JW, McColl SR, Kochetkova M
Molecular cancer 2015 Jun 6;14:115

---

Leukocyte attraction by CCL20 and its receptor CCR6 in humans and mice with pneumococcal meningitis.

Klein M, Brouwer MC, Angele B, Geldhoff M, Marquez G, Varona R, Häcker G, Schmetzer H, Häcker H, Hammerschmidt S, van der Ende A, Pfister HW, van de Beek D, Koedel U
PloS one 2014;9(4):e93057

---

Cryptococcus gattii infection dampens Th1 and Th17 responses by attenuating dendritic cell function and pulmonary chemokine expression in the immunocompetent hosts.

Angkasekwinai P, Sringkarin N, Supasorn O, Fungkrajai M, Wang YH, Chayakulkeeree M, Ngamskulrungroj P, Angkasekwinai N, Pattanapanyasat K
Infection and immunity 2014 Sep;82(9):3880-90

---

Differential developmental requirement and peripheral regulation for dermal Vγ4 and Vγ6T17 cells in health and inflammation.

Cai Y, Xue F, Fleming C, Yang J, Ding C, Ma Y, Liu M, Zhang HG, Zheng J, Xiong N, Yan J
Nature communications 2014 Jun 9;5:3986

---

Liver-resident NK cells confer adaptive immunity in skin-contact inflammation.

Peng H, Jiang X, Chen Y, Sojka DK, Wei H, Gao X, Sun R, Yokoyama WM, Tian Z
The Journal of clinical investigation 2013 Apr;123(4):1444-56

---

Difference in Th1 and Th17 lymphocyte adhesion to endothelium.

Alcaide P, Maganto-Garcia E, Newton G, Travers R, Croce KJ, Bu DX, Luscinskas FW, Lichtman AH
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 1;188(3):1421-30

---

Notch, Id2, and RORγt sequentially orchestrate the fetal development of lymphoid tissue inducer cells.

Cherrier M, Sawa S, Eberl G
The Journal of experimental medicine 2012 Apr 9;209(4):729-40

---

ATP drives lamina propria T(H)17 cell differentiation.

Atarashi K, Nishimura J, Shima T, Umesaki Y, Yamamoto M, Onoue M, Yagita H, Ishii N, Evans R, Honda K, Takeda K
Nature 2008 Oct 9;455(7214):808-12

---

Both Th1 and Th17 are immunopathogenic but differ in other key biological activities.

Cox CA, Shi G, Yin H, Vistica BP, Wawrousek EF, Chan CC, Gery I
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7414-22

---

Chemokine receptor CCR2 but not CCR5 or CCR6 mediates the increase in pulmonary dendritic cells during allergic airway inflammation.

Robays LJ, Maes T, Lebecque S, Lira SA, Kuziel WA, Brusselle GG, Joos GF, Vermaelen KV
Journal of immunology (Baltimore, Md. : 1950) 2007 Apr 15;178(8):5305-11

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of CCR6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Human/Mouse CCR6 PE-conjugated Monoclonal Antibody (Catalog # FAB590P) or (B) Rat IgG_2A Phycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of CCR6 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Rat Anti-Human/Mouse CCR6 PE-conjugated Monoclonal Antibody (Catalog # FAB590P) or (B) Rat IgG_2A Phycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.