FAB590T-100UG
antibody from R&D Systems
Targeting: CCR6
BN-1, CD196, CKR-L3, CMKBR6, DCR2, DRY-6, GPR-CY4, GPR29, STRL22
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB590T-100UG - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCR6 Alexa Fluor® 594-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CCR6-transfected rat RBL cells but not the parent cell line or cells transfected with mouse CCR5.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Red dye
- Antigen sequence
O54689
- Isotype
- IgG
- Antibody clone number
- 140706
- Vial size
- 100 ug
- Concentration
- 0.2 mg/ml
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CCR6 in Mouse Th17-Differentiated Splenocytes by Flow Cytometry. Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3 epsilon monoclonal antibody (Catalog # MAB484, 5 μg/mL) plus Goat anti-Mouse CD28 (Catalog # AF483, 5 μg/mL), Recombinant Human TGF- beta 1 (Catalog # 240-B, 10 ng/mL) Recombinant Mouse IL-23 (Catalog # 1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (Catalog # 406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (Catalog # 401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (Catalog # MAB485, 10 μg/mL) for 5 days were stained with (A) Rat Anti-Mouse CCR6 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB590T) or (B) RatIgG2A isotype control antibody (Catalog # IC006T) and Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). View our protocol for Staining Membrane-associated Proteins.