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- References [20]
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- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 15549-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15549-1-AP, RRID:AB_10694269
- Product name
- PRPS1 antibody
- Antibody type
- Polyclonal
- Description
- PRPS1 antibody (Cat. #15549-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Mechanistic insights into metformin's anti-hyperuricemic effect: Targeting PPP/DNPB/XOD-mediated purine pathway, purinosome assembly, and gut microbiota homostasis in rats.
The role of gene duplication and paralog specialisation in the evolution of the mammalian PRPS complex.
Depletion of nuclear cytoophidia in Alzheimer's disease.
PRPS activity tunes redox homeostasis in Myc-driven lymphoma.
NDUFS3 promotes proliferation via glucose metabolism reprogramming inducing AMPK phosphorylating PRPS1 to increase the purine nucleotide synthesis in melanoma.
NUDT5 regulates purine metabolism and thiopurine sensitivity by interacting with PPAT.
Evolutionary origins and innovations sculpting the mammalian PRPS enzyme complex.
De novo and salvage purine synthesis pathways across tissues and tumors.
Direct stimulation of de novo nucleotide synthesis by O-GlcNAcylation.
CRISPR/Cas9-Mediated Induction of Relapse-Specific NT5C2 and PRPS1 Mutations Confers Thiopurine Resistance as a Relapsed Lymphoid Leukemia Model.
NRF2-directed PRPS1 upregulation to promote the progression and metastasis of melanoma.
Identification of purine biosynthesis as an NADH-sensing pathway to mediate energy stress.
Molecular crowding facilitates bundling of IMPDH polymers and cytoophidium formation.
Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.
NOTCH1-driven UBR7 stimulates nucleotide biosynthesis to promote T cell acute lymphoblastic leukemia.
Mitochondrial NADP(+) is essential for proline biosynthesis during cell growth.
Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.
CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.
mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.
Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.
Yu Q, Liao H, Tan W, Xu L, Lin G, Ai Z, Xie Q, Liu Y, Li Y, Yang X, Su Z, Ma G, Xie J, Lai Z
Chemico-biological interactions 2025 Nov 1;421:111783
Chemico-biological interactions 2025 Nov 1;421:111783
The role of gene duplication and paralog specialisation in the evolution of the mammalian PRPS complex.
Karki BR, MacMillan AC, Vicente-Muñoz S, Greis KD, Romick LE, Cunningham JT
Nature communications 2025 Jul 8;16(1):6076
Nature communications 2025 Jul 8;16(1):6076
Depletion of nuclear cytoophidia in Alzheimer's disease.
Ivanova A, Munoz DG, Woulfe J
Free neuropathology 2025 Jan;6:8
Free neuropathology 2025 Jan;6:8
PRPS activity tunes redox homeostasis in Myc-driven lymphoma.
MacMillan AC, Karki B, Yang J, Gertz KR, Zumwalde S, Patel JG, Czyzyk-Krzeska MF, Meller J, Cunningham JT
bioRxiv : the preprint server for biology 2025 Feb 28;
bioRxiv : the preprint server for biology 2025 Feb 28;
NDUFS3 promotes proliferation via glucose metabolism reprogramming inducing AMPK phosphorylating PRPS1 to increase the purine nucleotide synthesis in melanoma.
Xiong G, Yun F, Jiang L, Yi Z, Yi X, Yang L, Zhang X, Li X, Yang Z, Zhang Q, Sai B, Kuang Y, Zhu Y
Cell death and differentiation 2025 Dec;32(12):2193-2209
Cell death and differentiation 2025 Dec;32(12):2193-2209
NUDT5 regulates purine metabolism and thiopurine sensitivity by interacting with PPAT.
Wu Z, Nguyen PT, Sondhi V, Yao RW, Lu Z, Dai T, Chiang JC, Cai F, Williams IM, Blatt EB, Shang Z, Cai L, Zhang J, Moore MD, Alshamleh I, Li X, Ogu T, Zacharias LG, Winston R, Patricio JS, Johnson X, Chen WM, Cong Q, Mathews TP, Zhang Y, Zhang L, DeBerardinis RJ
Science (New York, N.Y.) 2025 Dec 11;390(6778):1134-1142
Science (New York, N.Y.) 2025 Dec 11;390(6778):1134-1142
Evolutionary origins and innovations sculpting the mammalian PRPS enzyme complex.
Karki BR, Macmillan AC, Vicente-Muñoz S, Greis KD, Romick LE, Cunningham JT
bioRxiv : the preprint server for biology 2024 Oct 1;
bioRxiv : the preprint server for biology 2024 Oct 1;
De novo and salvage purine synthesis pathways across tissues and tumors.
Tran DH, Kim D, Kesavan R, Brown H, Dey T, Soflaee MH, Vu HS, Tasdogan A, Guo J, Bezwada D, Al Saad H, Cai F, Solmonson A, Rion H, Chabatya R, Merchant S, Manales NJ, Tcheuyap VT, Mulkey M, Mathews TP, Brugarolas J, Morrison SJ, Zhu H, DeBerardinis RJ, Hoxhaj G
Cell 2024 Jul 11;187(14):3602-3618.e20
Cell 2024 Jul 11;187(14):3602-3618.e20
Direct stimulation of de novo nucleotide synthesis by O-GlcNAcylation.
Chen L, Zhou Q, Zhang P, Tan W, Li Y, Xu Z, Ma J, Kupfer GM, Pei Y, Song Q, Pei H
Nature chemical biology 2024 Jan;20(1):19-29
Nature chemical biology 2024 Jan;20(1):19-29
CRISPR/Cas9-Mediated Induction of Relapse-Specific NT5C2 and PRPS1 Mutations Confers Thiopurine Resistance as a Relapsed Lymphoid Leukemia Model.
Nguyen TTT, Tanaka Y, Sanada M, Hosaka M, Tamai M, Kagami K, Komatsu C, Somazu S, Harama D, Kasai S, Watanabe A, Akahane K, Goi K, Inukai T
Molecular pharmacology 2023 Apr;103(4):199-210
Molecular pharmacology 2023 Apr;103(4):199-210
NRF2-directed PRPS1 upregulation to promote the progression and metastasis of melanoma.
Xiong G, Feng Y, Yi X, Zhang X, Li X, Yang L, Yi Z, Sai B, Yang Z, Zhang Q, Kuang Y, Zhu Y
Frontiers in immunology 2022;13:989263
Frontiers in immunology 2022;13:989263
Identification of purine biosynthesis as an NADH-sensing pathway to mediate energy stress.
Yang R, Yang C, Ma L, Zhao Y, Guo Z, Niu J, Chu Q, Ma Y, Li B
Nature communications 2022 Nov 17;13(1):7031
Nature communications 2022 Nov 17;13(1):7031
Molecular crowding facilitates bundling of IMPDH polymers and cytoophidium formation.
Chang CC, Peng M, Zhong J, Zhang Z, Keppeke GD, Sung LY, Liu JL
Cellular and molecular life sciences : CMLS 2022 Jul 14;79(8):420
Cellular and molecular life sciences : CMLS 2022 Jul 14;79(8):420
Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.
Chen X, Wu M, Liang N, Lu J, Qu S, Chen H
Frontiers in cell and developmental biology 2021;9:636802
Frontiers in cell and developmental biology 2021;9:636802
NOTCH1-driven UBR7 stimulates nucleotide biosynthesis to promote T cell acute lymphoblastic leukemia.
Srivastava S, Sahu U, Zhou Y, Hogan AK, Sathyan KM, Bodner J, Huang J, Wong KA, Khalatyan N, Savas JN, Ntziachristos P, Ben-Sahra I, Foltz DR
Science advances 2021 Jan;7(5)
Science advances 2021 Jan;7(5)
Mitochondrial NADP(+) is essential for proline biosynthesis during cell growth.
Tran DH, Kesavan R, Rion H, Soflaee MH, Solmonson A, Bezwada D, Vu HS, Cai F, Phillips JA 3rd, DeBerardinis RJ, Hoxhaj G
Nature metabolism 2021 Apr;3(4):571-585
Nature metabolism 2021 Apr;3(4):571-585
Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.
Gu Z, Zhang H, Guo X, Cao Y
The Journal of investigative dermatology 2020 Aug;140(8):1513-1523.e5
The Journal of investigative dermatology 2020 Aug;140(8):1513-1523.e5
CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.
Gu Z, Zhang H, Li Y, Shen S, Yin X, Zhang W, Cheng R, Zhang Y, Zhang X, Chen H, Huang B, Cao Y
Oncotarget 2017 Dec 26;8(69):114328-114343
Oncotarget 2017 Dec 26;8(69):114328-114343
mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.
Ben-Sahra I, Hoxhaj G, Ricoult SJH, Asara JM, Manning BD
Science (New York, N.Y.) 2016 Feb 12;351(6274):728-733
Science (New York, N.Y.) 2016 Feb 12;351(6274):728-733
Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.
Bai C, Tang S, Bai C, Chen X
Amino acids 2014 Apr;46(4):841-52
Amino acids 2014 Apr;46(4):841-52
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15549-1-AP(PRPS1 antibody) at dilution of 1:100
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using PRPS1 antibody 15549-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human testis using 15549-1-AP(PRPS1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human testis using 15549-1-AP(PRPS1 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue
