Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX132110 - Provider product page
- Provider
- GeneTex
- Product name
- HMGA1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody (GTX132110) diluted at 1:3000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 £gg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody (GTX132110) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody (GTX132110) diluted at 1:3000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGA1 antibody detects HMGA1 protein at nucleus by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGA1 protein stained by HMGA1 antibody (GTX132110) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.