MA5-56560

antibody from Invitrogen Antibodies

Targeting: HMGA1 HMGIY

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-56560

Provider

Invitrogen Antibodies

Product name

HMGA1 Recombinant Rabbit Monoclonal Antibody (JE58-17)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JE58-17

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of HMGA1 with HCT 116 cells. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp). Next they were incubated with HMGA1 Recombinant Monoclonal Antibody (Product # MA5-56560)at a dilution of 1:200 in 1% BSA in PBST overnight at 4 ℃. Followed by Goat Anti-Rabbit IgG H&L at a dilution of 1:1,000. DAPI was used to stain the cell nuclei (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HMGA1 with HCT 116 cells. The cells were fixed, permeabilized and incubated with HMGA1 Recombinant Monoclonal Antibody (Product # MA5-56560) at a dilution of 1:1,000 (red) and compared with Rabbit IgG Isotype Control (green). Followed by iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1:1,000 dilution (30 min, 4℃).