PA5-56236

antibody from Invitrogen Antibodies

Targeting: RAD23B HHR23B, HR23B, P58

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-56236

Provider

Invitrogen Antibodies

Product name

RAD23B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: VIEALRASFN NPDRAVEYLL MGIPGDRESQ AVVDPPQAAS TGAPQSSAVA AAAATTTATT TTTSSGGHPL EFLRNQPQFQ QMRQIIQQNP Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 93%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RAD23B in control (vector only transfected HEK293T lysate) and RAD23B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a RAD23B Polyclonal Antibody (Product # PA5-56236).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RAD23B in human cell line RH-30 using a RAD23B Polyclonal Antibody (Product # PA5-56236).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RAD23B in control (vector only transfected HEK293T lysate) and RAD23B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a RAD23B Polyclonal Antibody (Product # PA5-56236).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of RAD23B in human cell line U-251 MG shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a RAD23B Polyclonal Antibody (Product # PA5-56236).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of RAD23B in human cell line A-431 using a RAD23B Polyclonal Antibody (Product # PA5-56236) shows localization to nucleoplasm and cytosol.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of RAD23B in human colon using RAD23B Polyclonal Antibody (Product # PA5-56236).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of RAD23B in human liver using RAD23B Polyclonal Antibody (Product # PA5-56236).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of RAD23B in human testis using RAD23B Polyclonal Antibody (Product # PA5-56236).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of RAD23B in human kidney using RAD23B Polyclonal Antibody (Product # PA5-56236).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of RAD23B in human colon, kidney, liver and testis using RAD23B Polyclonal Antibody (Product # PA5-56236) (A) shows similar protein distribution across tissues to an independent RAD23B Polyclonal Antibody (B).