MA5-50606

antibody from Invitrogen Antibodies

Targeting: TMED9 HSGP25L2G, p24a2, p24alpha2

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-50606

Provider

Invitrogen Antibodies

Product name

TMED9 Monoclonal Antibody (A9A7)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive control: Hela cell lysate, THP-1 cell lysate, A549 cell lysate, MCF-7 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, mouse lung tissue lysate, HepG2 cell lysate, LO2 cell lysate, human liver tissue lysate, rat liver tissue lysate, mouse liver tissue. Predicted band size: 27 kDa Subcellular Location: Endoplasmic reticulum membrane, Golgi apparatus, Endoplasmic reticulum-Golgi intermediate compartment membrane.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

A9A7

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of TMED9 in paraffin-embedded mouse liver tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with TMED9 monoclonal antibody (Product # MA5-50606) with a dilution of 1:1,000 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.