TA303362

antibody from OriGene

Targeting: HCRTR1 OX1R

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA303362

Provider

OriGene

Proper citation

OriGene Cat#TA303362, RRID:AB_2117667

Product name

Goat Polyclonal Antibody against HCRTR1

Antibody type

Polyclonal

Description

Goat Polyclonal Antibody against HCRTR1

Host

Goat

Conjugate

Unconjugated

Epitope

HCRTR1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

NULL

References

Submitted references

Hypocretin (orexin) regulates glutamate input to fast-spiking interneurons in layer V of the Fr2 region of the murine prefrontal cortex.

Aracri P, Banfi D, Pasini ME, Amadeo A, Becchetti A
Cerebral cortex (New York, N.Y. : 1991) 2015 May;25(5):1330-47

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

TA303362 (0.5ug/ml) staining of Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Figure from citation: Western Blot of HCRTR1 protein level by using anti-HCRTR1 antibody in mouse membrane fractions. Dilution: 1:500

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

ICC analysis of HCRTR-1 expression by using anti-HCRTR1 antibody. In layer V of SS (C), the HCRTR-1 granular immunoreactivity was detected at the level of neuronal cell bodies based on morphology (arrows). (C') gives a higher magnification detail of (C). In layer V of Fr2 (D), the HCRTR-1 positive (+) signal was more intense than in SS(arrows and inset in D'), but also in neuropilar processes of different caliber (arrowheads). (D') gives a higher magnification detail of (C). Dilution: 1:150

Validation comment

IF