Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
Reference
Comment
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- Product number
- TA303362 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA303362, RRID:AB_2117667
- Product name
- Goat Polyclonal Antibody against HCRTR1
- Antibody type
- Polyclonal
- Description
- Goat Polyclonal Antibody against HCRTR1
- Host
- Goat
- Conjugate
- Unconjugated
- Epitope
- HCRTR1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- NULL
Submitted references Hypocretin (orexin) regulates glutamate input to fast-spiking interneurons in layer V of the Fr2 region of the murine prefrontal cortex.
Aracri P, Banfi D, Pasini ME, Amadeo A, Becchetti A
Cerebral cortex (New York, N.Y. : 1991) 2015 May;25(5):1330-47
Cerebral cortex (New York, N.Y. : 1991) 2015 May;25(5):1330-47
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- TA303362 (0.5ug/ml) staining of Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western Blot of HCRTR1 protein level by using anti-HCRTR1 antibody in mouse membrane fractions. Dilution: 1:500
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ICC analysis of HCRTR-1 expression by using anti-HCRTR1 antibody. In layer V of SS (C), the HCRTR-1 granular immunoreactivity was detected at the level of neuronal cell bodies based on morphology (arrows). (C') gives a higher magnification detail of (C). In layer V of Fr2 (D), the HCRTR-1 positive (+) signal was more intense than in SS(arrows and inset in D'), but also in neuropilar processes of different caliber (arrowheads). (D') gives a higher magnification detail of (C). Dilution: 1:150
- Validation comment
- IF