PA5-77566

antibody from Invitrogen Antibodies

Targeting: HCRTR1 OX1R

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-77566

Provider

Invitrogen Antibodies

Product name

Orexin Receptor 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

50 µL

Concentration

0.8 mg/mL

Storage

-20°C

References

Submitted references

Orexin A alleviates neuroinflammation via OXR2/CaMKKβ/AMPK signaling pathway after ICH in mice.

Li T, Xu W, Ouyang J, Lu X, Sherchan P, Lenahan C, Irio G, Zhang JH, Zhao J, Zhang Y, Tang J
Journal of neuroinflammation 2020 Jun 15;17(1):187

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lines with Orexin Receptor 1 polyclonal antibody (Product # PA5-77566) using a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lines with Orexin Receptor 1 polyclonal antibody (Product # PA5-77566) using a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lines with Orexin Receptor 1 polyclonal antibody (Product # PA5-77566) using a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lines with Orexin Receptor 1 polyclonal antibody (Product # PA5-77566) using a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Orexin Receptor 1 in paraformaldehyde-fixed and permeabilized human colon cancer cells. A) Samples were probed with Orexin Receptor 1 polyclonal antibody (Product # PA5-77566) at a dilution of 1:200, and incubated with goat-anti-rabbit-AlexaFluor-555 and Hoechst. B) Shows live view of samples. C) Shows nuclei stained image.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Orexin Receptor 1 in frozen rat brainstem. Samples were probed with Orexin Receptor 1 polyclonal antibody (Product # PA5-77566) using a dilution of 1:50, and incubated with goat-anti-rabbit-AlexaFluor-555 and Hoechst.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Orexin Receptor 1 in frozen rat brainstem. Samples were probed with Orexin Receptor 1 polyclonal antibody (Product # PA5-77566) using a dilution of 1:50, and incubated with goat-anti-rabbit-AlexaFluor-555 and Hoechst.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Orexin Receptor 1 in frozen rat brainstem. Samples were probed with Orexin Receptor 1 polyclonal antibody (Product # PA5-77566) using a dilution of 1:50, and incubated with goat-anti-rabbit-AlexaFluor-555 and Hoechst.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 Time-course study of OXA, OXR1, and OXR2 using western blot. a Representative protein bands. b Western blot quantitative analyses of OXA, one-way ANOVA, * p < 0.05 vs. sham group. Error bars represent the means +- SD, n = 6 per group. c Western blot quantitative analyses of OXR1, one-way ANOVA, * p < 0.05 vs. sham group. Error bars represent the means +- SD, n = 6 per group. d Western blot quantitative analyses of OXR2, one-way ANOVA, * p < 0.05 vs. sham group. Error bars represent mean +- SD, n = 6 per group