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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AMR-023-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MC3 Receptor (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-MC3 Receptor (extracellular) Antibody (#AMR-023), (1:200).3,4. Anti-MC3 Receptor (extracellular) Antibody , preincubated with MC3 Receptor (extracellular) Blocking Peptide (#BLP-MR023).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-MC3 Receptor (extracellular) Antibody (#AMR-023), (1:200).3,4. Anti-MC3 Receptor (extracellular) Antibody , preincubated with MC3 Receptor (extracellular) Blocking Peptide (#BLP-MR023).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of MC3R in rat brain - Immunohistochemical staining of perfusion-fixed frozen rat brainsections using Anti-MC3 Receptor (extracellular) Antibody (#AMR-023), (1:100). Expression (green) appears in the hypothalamic periventricular region (horizontal arrow), in some nerve cells (vertical arrow), and in the neuropil (asterisk). Hoechst 33342 is used as the counterstain (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of MC3R by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-MC3 Receptor (extracellular) Antibody (#AMR-023), (2.5μg) + goat- Anti-rabbit-FITC.
