Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [4]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-97953 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 2.775 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot analysis of MAS1 using a MAS1 Polyclonal antibody (Product # PA5-97953) at a concentration of 2 µg/mL. Lane 1: 293T whole cell lysate. Lane 2: Colo320 whole cell lysate. Lane 3: HepG2 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:10,000 dilution. Observed band size: 38 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of MAS1 in mouse brain tissue using a MAS1 polyclonal antibody (Product # PA5-97953) at a dilution of 1:2,000. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MAS1 in HepG2 cells using a MAS1 polyclonal antibody (Product # PA5-97953) at a dilution of 1:100. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibody was used.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MAS1 in Hela cells using a MAS1 polyclonal antibody (Product # PA5-97953) at a dilution of 1:100. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibody was used.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MAS1 in HepG2 cells using a MAS1 polyclonal antibody (Product # PA5-97953) at a dilution of 1:100. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibody was used.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of MAS1 in Hela cells. The sample was incubated with a MAS1 polyclonal antibody (Product # PA5-97953) at a dilution of 1:200, followed by Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MAS1 in paraffin embedded human kidney tissue using a MAS1 polyclonal antibody (Product # PA5-97953) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MAS1 in paraffin embedded human testis tissue using a MAS1 polyclonal antibody (Product # PA5-97953) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of MAS1 in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT, then the primary antibody (1% BSA) was incubated at 4°C overnight. The sample was incubated with a MAS1 polyclonal antibody (Product # PA5-97953) at a dilution of 1:200, followed by a goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.