Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- PPS073 - Provider product page
- Provider
- R&D Systems
- Product name
- Phospho-GABA-B R2 (S892) Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Human, mouse, rat, and Xenopus ~110 kDa GABAB Receptor, R2 subunit phosphorylated at S892
- Reactivity
- Human, Mouse, Rat, Xenopus
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage,
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Phospho-GABAB R2 (S892) by Western Blot Western blots of COS-7 cells transiently expressing mutant GABAB R2 (S892A) (lane 1), wild type GABAB R2 (lane 2) or control untransfected cells (lane 3) were lysed and 50 mg of protein from each sample was separated by SDS-PAGE and transferred to a nitrocellulose membrane. Blots were then immunolabeled with rabbit anti-phospho-GABAB R2 (S892) (A). In B, the membrane was pre-treated with lambda-PPase (1200 units for 30 minutes) before being exposed to the anti-GABAB Receptor, R2 subunit (S892). The immunolabeling is completely eliminated by treatment with lambda-PPase.