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LearnNB100-61596
antibody from Novus Biologicals
Targeting: CPSF6
CFIM, CFIM68, CFIM72, HPBRII-4, HPBRII-7
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- NB100-61596 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-61596, RRID:AB_963044
- Product name
- Rabbit Polyclonal CPSF6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Capsid-CPSF6 Interaction Is Dispensable for HIV-1 Replication in Primary Cells but Is Selected during Virus Passage In Vivo.
In vivo functions of CPSF6 for HIV-1 as revealed by HIV-1 capsid evolution in HLA-B27-positive subjects.
The ability of TNPO3-depleted cells to inhibit HIV-1 infection requires CPSF6.
Saito A, Henning MS, Serrao E, Dubose BN, Teng S, Huang J, Li X, Saito N, Roy SP, Siddiqui MA, Ahn J, Tsuji M, Hatziioannou T, Engelman AN, Yamashita M
Journal of virology 2016 Aug 1;90(15):6918-6935
Journal of virology 2016 Aug 1;90(15):6918-6935
In vivo functions of CPSF6 for HIV-1 as revealed by HIV-1 capsid evolution in HLA-B27-positive subjects.
Henning MS, Dubose BN, Burse MJ, Aiken C, Yamashita M
PLoS pathogens 2014 Jan;10(1):e1003868
PLoS pathogens 2014 Jan;10(1):e1003868
The ability of TNPO3-depleted cells to inhibit HIV-1 infection requires CPSF6.
Fricke T, Valle-Casuso JC, White TE, Brandariz-Nuñez A, Bosche WJ, Reszka N, Gorelick R, Diaz-Griffero F
Retrovirology 2013 Apr 26;10:46
Retrovirology 2013 Apr 26;10:46
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: CPSF6 Antibody [NB100-61596] - Simple Western lane view shows a specific band for CPSF6 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CPSF6 Antibody [NB100-61596] - Detection of Human and Mouse CPSF68 on HeLa whole cell lysate using NB100-61596. CPSF68 was also immunoprecipitated by rabbit anti-CPSF68 antibodies NB100-61597 and NB100-61598.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CPSF6 Antibody [NB100-61596] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiCPSF68 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CPSF6 Antibody [NB100-61596] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CPSF68 used at a dilution of 1:200 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CPSF6 Antibody [NB100-61596] - FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CPSF68 used at a dilution of 1:200 (1ug/ml). Detection: DAB
