Antibody data
- Antibody Data
- Antigen structure
- References [25]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- 12201-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12201-1-AP, RRID:AB_2220710
- Product name
- ABHD5 antibody
- Antibody type
- Polyclonal
- Description
- ABHD5 antibody (Cat. #12201-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references The constitutively active form of a key cholesterol synthesis enzyme is lipid droplet-localized and upregulated in endometrial cancer tissues.
Parallel CRISPR-Cas9 screens identify mechanisms of PLIN2 and lipid droplet regulation.
Miquelianin in Folium Nelumbinis extract promotes white-to-beige fat conversion via blocking AMPK/DRP1/mitophagy and modulating gut microbiota in HFD-fed mice.
Aging reduces ABHD5 protein content in the adipose tissue of mice: The reversal effect of exercise.
Plin2-mediated lipid droplet mobilization accelerates exit from pluripotency by lipidomic remodeling and histone acetylation.
Endurance exercise-mediated metabolic reshuffle attenuates high-caloric diet-induced non-alcoholic fatty liver disease.
Strength training alters the tissue fatty acids profile and slightly improves the thermogenic pathway in the adipose tissue of obese mice.
ABHD5 suppresses cancer cell anabolism through lipolysis-dependent activation of the AMPK/mTORC1 pathway.
Prolyl isomerase Pin1 interacts with adipose triglyceride lipase and negatively controls both its expression and lipolysis.
Peroxisomal β-oxidation acts as a sensor for intracellular fatty acids and regulates lipolysis.
ORP5 localizes to ER-lipid droplet contacts and regulates the level of PI(4)P on lipid droplets.
O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity.
Dynamic interactions of ABHD5 with PNPLA3 regulate triacylglycerol metabolism in brown adipocytes.
Ces1d deficiency protects against high-sucrose diet-induced hepatic triacylglycerol accumulation.
Maternal obesity aggravates the abnormality of porcine placenta by increasing N(6)-methyladenosine.
Inhibition of intracellular lipolysis promotes human cancer cell adaptation to hypoxia.
Regulation of G0/G1 Switch Gene 2 (G0S2) Protein Ubiquitination and Stability by Triglyceride Accumulation and ATGL Interaction.
CDP-diacylglycerol synthases regulate the growth of lipid droplets and adipocyte development.
Pleiotropic effects of cavin-1 deficiency on lipid metabolism.
Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis.
Regulation of FSP27 protein stability by AMPK and HSC70.
Distinct mechanisms regulate ATGL-mediated adipocyte lipolysis by lipid droplet coat proteins.
Spatial regulation of UBXD8 and p97/VCP controls ATGL-mediated lipid droplet turnover.
Relative contribution of adipose triglyceride lipase and hormone-sensitive lipase to tumor necrosis factor-α (TNF-α)-induced lipolysis in adipocytes.
Functional interactions between Mldp (LSDP5) and Abhd5 in the control of intracellular lipid accumulation.
Coates HW, Nguyen TB, Du X, Olzomer EM, Farrell R, Byrne FL, Yang H, Brown AJ
The Journal of biological chemistry 2024 May;300(5):107232
The Journal of biological chemistry 2024 May;300(5):107232
Parallel CRISPR-Cas9 screens identify mechanisms of PLIN2 and lipid droplet regulation.
Roberts MA, Deol KK, Mathiowetz AJ, Lange M, Leto DE, Stevenson J, Hashemi SH, Morgens DW, Easter E, Heydari K, Nalls MA, Bassik MC, Kampmann M, Kopito RR, Faghri F, Olzmann JA
Developmental cell 2023 Sep 25;58(18):1782-1800.e10
Developmental cell 2023 Sep 25;58(18):1782-1800.e10
Miquelianin in Folium Nelumbinis extract promotes white-to-beige fat conversion via blocking AMPK/DRP1/mitophagy and modulating gut microbiota in HFD-fed mice.
Wang Z, Yang T, Zeng M, Wang Z, Chen Q, Chen J, Christian M, He Z
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2023 Nov;181:114089
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2023 Nov;181:114089
Aging reduces ABHD5 protein content in the adipose tissue of mice: The reversal effect of exercise.
Brícola RS, Cordeiro AV, Crisol BM, Braga RR, de Melo DG, Rocha MB, Gaspar RC, Nakandakari SCBR, Silva VRR, Anaruma CP, Katashima CK, Canciglieri RDS, Munõz VR, Pavan ICB, Pinto AP, Simabuco FM, Silva ASRD, Moura LP, Pauli JR, Cintra DE, Ropelle ER
Cell biochemistry and function 2023 Jan;41(1):128-137
Cell biochemistry and function 2023 Jan;41(1):128-137
Plin2-mediated lipid droplet mobilization accelerates exit from pluripotency by lipidomic remodeling and histone acetylation.
Wu Y, Chen K, Li L, Hao Z, Wang T, Liu Y, Xing G, Liu Z, Li H, Yuan H, Lu J, Zhang C, Zhang J, Zhao D, Wang J, Nie J, Ye D, Pan G, Chan WY, Liu X
Cell death and differentiation 2022 Nov;29(11):2316-2331
Cell death and differentiation 2022 Nov;29(11):2316-2331
Endurance exercise-mediated metabolic reshuffle attenuates high-caloric diet-induced non-alcoholic fatty liver disease.
Cook JJ, Wei M, Segovia B, Cosio-Lima L, Simpson J, Taylor S, Koh Y, Kim S, Lee Y
Annals of hepatology 2022 Jul-Aug;27(4):100709
Annals of hepatology 2022 Jul-Aug;27(4):100709
Strength training alters the tissue fatty acids profile and slightly improves the thermogenic pathway in the adipose tissue of obese mice.
de Melo DG, Anaruma CP, da Cruz Rodrigues KC, Pereira RM, de Campos TDP, Canciglieri RS, Ramos CO, Cintra DE, Ropelle ER, da Silva ASR, Pauli JR, de Moura LP
Scientific reports 2022 Apr 28;12(1):6913
Scientific reports 2022 Apr 28;12(1):6913
ABHD5 suppresses cancer cell anabolism through lipolysis-dependent activation of the AMPK/mTORC1 pathway.
Chen G, Zhou G, Lotvola A, Granneman JG, Wang J
The Journal of biological chemistry 2021 Jan-Jun;296:100104
The Journal of biological chemistry 2021 Jan-Jun;296:100104
Prolyl isomerase Pin1 interacts with adipose triglyceride lipase and negatively controls both its expression and lipolysis.
Nakatsu Y, Yamamotoya T, Okumura M, Ishii T, Kanamoto M, Naito M, Nakanishi M, Aoyama S, Matsunaga Y, Kushiyama A, Sakoda H, Fujishiro M, Ono H, Asano T
Metabolism: clinical and experimental 2021 Feb;115:154459
Metabolism: clinical and experimental 2021 Feb;115:154459
Peroxisomal β-oxidation acts as a sensor for intracellular fatty acids and regulates lipolysis.
Ding L, Sun W, Balaz M, He A, Klug M, Wieland S, Caiazzo R, Raverdy V, Pattou F, Lefebvre P, Lodhi IJ, Staels B, Heim M, Wolfrum C
Nature metabolism 2021 Dec;3(12):1648-1661
Nature metabolism 2021 Dec;3(12):1648-1661
ORP5 localizes to ER-lipid droplet contacts and regulates the level of PI(4)P on lipid droplets.
Du X, Zhou L, Aw YC, Mak HY, Xu Y, Rae J, Wang W, Zadoorian A, Hancock SE, Osborne B, Chen X, Wu JW, Turner N, Parton RG, Li P, Yang H
The Journal of cell biology 2020 Jan 6;219(1)
The Journal of cell biology 2020 Jan 6;219(1)
O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity.
Yang Y, Fu M, Li MD, Zhang K, Zhang B, Wang S, Liu Y, Ni W, Ong Q, Mi J, Yang X
Nature communications 2020 Jan 10;11(1):181
Nature communications 2020 Jan 10;11(1):181
Dynamic interactions of ABHD5 with PNPLA3 regulate triacylglycerol metabolism in brown adipocytes.
Yang A, Mottillo EP, Mladenovic-Lucas L, Zhou L, Granneman JG
Nature metabolism 2019 May;1(5):560-569
Nature metabolism 2019 May;1(5):560-569
Ces1d deficiency protects against high-sucrose diet-induced hepatic triacylglycerol accumulation.
Lian J, Watts R, Quiroga AD, Beggs MR, Alexander RT, Lehner R
Journal of lipid research 2019 Apr;60(4):880-891
Journal of lipid research 2019 Apr;60(4):880-891
Maternal obesity aggravates the abnormality of porcine placenta by increasing N(6)-methyladenosine.
Song T, Lu J, Deng Z, Xu T, Yang Y, Wei H, Li S, Jiang S, Peng J
International journal of obesity (2005) 2018 Oct;42(10):1812-1820
International journal of obesity (2005) 2018 Oct;42(10):1812-1820
Inhibition of intracellular lipolysis promotes human cancer cell adaptation to hypoxia.
Zhang X, Saarinen AM, Hitosugi T, Wang Z, Wang L, Ho TH, Liu J
eLife 2017 Dec 19;6
eLife 2017 Dec 19;6
Regulation of G0/G1 Switch Gene 2 (G0S2) Protein Ubiquitination and Stability by Triglyceride Accumulation and ATGL Interaction.
Heckmann BL, Zhang X, Saarinen AM, Liu J
PloS one 2016;11(6):e0156742
PloS one 2016;11(6):e0156742
CDP-diacylglycerol synthases regulate the growth of lipid droplets and adipocyte development.
Qi Y, Kapterian TS, Du X, Ma Q, Fei W, Zhang Y, Huang X, Dawes IW, Yang H
Journal of lipid research 2016 May;57(5):767-80
Journal of lipid research 2016 May;57(5):767-80
Pleiotropic effects of cavin-1 deficiency on lipid metabolism.
Ding SY, Lee MJ, Summer R, Liu L, Fried SK, Pilch PF
The Journal of biological chemistry 2014 Mar 21;289(12):8473-83
The Journal of biological chemistry 2014 Mar 21;289(12):8473-83
Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis.
Patel S, Yang W, Kozusko K, Saudek V, Savage DB
Proceedings of the National Academy of Sciences of the United States of America 2014 Jun 24;111(25):9163-8
Proceedings of the National Academy of Sciences of the United States of America 2014 Jun 24;111(25):9163-8
Regulation of FSP27 protein stability by AMPK and HSC70.
Zhang X, Heckmann BL, Xie X, Saarinen AM, Liu J
American journal of physiology. Endocrinology and metabolism 2014 Dec 1;307(11):E1047-56
American journal of physiology. Endocrinology and metabolism 2014 Dec 1;307(11):E1047-56
Distinct mechanisms regulate ATGL-mediated adipocyte lipolysis by lipid droplet coat proteins.
Yang X, Heckmann BL, Zhang X, Smas CM, Liu J
Molecular endocrinology (Baltimore, Md.) 2013 Jan;27(1):116-26
Molecular endocrinology (Baltimore, Md.) 2013 Jan;27(1):116-26
Spatial regulation of UBXD8 and p97/VCP controls ATGL-mediated lipid droplet turnover.
Olzmann JA, Richter CM, Kopito RR
Proceedings of the National Academy of Sciences of the United States of America 2013 Jan 22;110(4):1345-50
Proceedings of the National Academy of Sciences of the United States of America 2013 Jan 22;110(4):1345-50
Relative contribution of adipose triglyceride lipase and hormone-sensitive lipase to tumor necrosis factor-α (TNF-α)-induced lipolysis in adipocytes.
Yang X, Zhang X, Heckmann BL, Lu X, Liu J
The Journal of biological chemistry 2011 Nov 25;286(47):40477-85
The Journal of biological chemistry 2011 Nov 25;286(47):40477-85
Functional interactions between Mldp (LSDP5) and Abhd5 in the control of intracellular lipid accumulation.
Granneman JG, Moore HH, Mottillo EP, Zhu Z
The Journal of biological chemistry 2009 Jan 30;284(5):3049-3057
The Journal of biological chemistry 2009 Jan 30;284(5):3049-3057
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 12201-1-AP(ABHD5 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using ABHD5 antibody 12201-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human skeletal muscle using 12201-1-AP(ABHD5 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human skeletal muscle using 12201-1-AP(ABHD5 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue