R32489

antibody from NSJ Bioreagents

Targeting: ABHD5 CGI-58, NCIE2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R32489

Provider

NSJ Bioreagents

Product name

ABHD5 Antibody / CGI-58

Antibody type

Polyclonal

Description

This highly specific ABHD5 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Immunocytochemistry applications with human and rat samples.

Reactivity

Human, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the ABHD5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat kidney and 2) human A431 lysate with ABHD5 antibody at 0.5ug/ml. Predicted molecular weight ~39 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IF/ICC staining of FFPE human A431 cells with ABHD5 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam sections in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human intestinal cancer tissue with ABHD5 antibody at 1 ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.