Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- MA5-24225 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATG4A Monoclonal Antibody (514914)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, no cross-reactivity with recombinant human ATG4B is observed.
- Antibody clone number
- 514914
- Concentration
- 0.5 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunocytochemistry analysis of ATG4A in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line. Samples were incubated in ATG4A monoclonal antibody (Product # MA5-24225) using a dilution of 25 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.