Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-57632 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATG4A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QSPQRMNILN LDPSVALGFF CKEEKDFDNW CSLVQKEILK ENLRMFELVQ KHPSHWPPFV PPAKPEVTTT GAEFIDSTEQ LEEFDLEEDF EILSV Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 91%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.7 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATG4A in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an ATG4A Polyclonal Antibody (Product # PA5-57632).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATG4A in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4. Samples were probed using an ATG4A Polyclonal Antibody (Product # PA5-57632).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ATG4A in human cell line A-431 shows positivity in nucleus, nucleoli, cytoplasm & microtubule organizing center. Samples were probed using an ATG4A Polyclonal Antibody (Product # PA5-57632).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ATG4A in human pancreas shows strong cytoplasmic positivity in exocrine glandular cells. Samples were probed using an ATG4A Polyclonal Antibody (Product # PA5-57632).
