Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 15669-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15669-1-AP, RRID:AB_2215019
- Product name
- VPS25 antibody
- Antibody type
- Polyclonal
- Description
- VPS25 antibody (Cat. #15669-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Lipid-anchored proteasomes control membrane protein homeostasis.
Enterovirus 71 non-structural protein 3A hijacks vacuolar protein sorting 25 to boost exosome biogenesis to facilitate viral replication.
Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.
YTHDC1-mediated VPS25 regulates cell cycle by targeting JAK-STAT signaling in human glioma cells.
Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.
VPS37A directs ESCRT recruitment for phagophore closure.
A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.
Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair.
Zhang R, Pan S, Zheng S, Liao Q, Jiang Z, Wang D, Li X, Hu A, Li X, Zhu Y, Shen X, Lei J, Zhong S, Zhang X, Huang L, Wang X, Huang L, Shen L, Song BL, Zhao JW, Wang Z, Yang B, Guo X
Science advances 2023 Dec;9(48):eadj4605
Science advances 2023 Dec;9(48):eadj4605
Enterovirus 71 non-structural protein 3A hijacks vacuolar protein sorting 25 to boost exosome biogenesis to facilitate viral replication.
Ruan Z, Liang Y, Chen Z, Yin J, Li C, Pan P, Zhang Q, Wu J, Luo Z
Frontiers in microbiology 2022;13:1024899
Frontiers in microbiology 2022;13:1024899
Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.
Liu CC, Liu YY, Zhou JF, Chen X, Chen H, Hu JH, Chen J, Zhang J, Sun RC, Wei JC, Go YY, Morita E, Zhou B
PLoS pathogens 2022 Feb;18(2):e1010294
PLoS pathogens 2022 Feb;18(2):e1010294
YTHDC1-mediated VPS25 regulates cell cycle by targeting JAK-STAT signaling in human glioma cells.
Zhu X, Yang H, Zhang M, Wu X, Jiang L, Liu X, Lv K
Cancer cell international 2021 Dec 4;21(1):645
Cancer cell international 2021 Dec 4;21(1):645
Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.
Mercier V, Larios J, Molinard G, Goujon A, Matile S, Gruenberg J, Roux A
Nature cell biology 2020 Aug;22(8):947-959
Nature cell biology 2020 Aug;22(8):947-959
VPS37A directs ESCRT recruitment for phagophore closure.
Takahashi Y, Liang X, Hattori T, Tang Z, He H, Chen H, Liu X, Abraham T, Imamura-Kawasawa Y, Buchkovich NJ, Young MM, Wang HG
The Journal of cell biology 2019 Oct 7;218(10):3336-3354
The Journal of cell biology 2019 Oct 7;218(10):3336-3354
A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.
Karasmanis EP, Hwang D, Nakos K, Bowen JR, Angelis D, Spiliotis ET
Current biology : CB 2019 Jul 8;29(13):2174-2182.e7
Current biology : CB 2019 Jul 8;29(13):2174-2182.e7
Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair.
Scheffer LL, Sreetama SC, Sharma N, Medikayala S, Brown KJ, Defour A, Jaiswal JK
Nature communications 2014 Dec 23;5:5646
Nature communications 2014 Dec 23;5:5646
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15669-1-AP(VPS25 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 15669-1-AP(VPS25 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The VPS25 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human VPS25. This antibody recognizes human, mouse, rat antigen. The VPS25 antibody has been validated for the following applications: ELISA, WB, IHC analysis.