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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- R35394 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- VPS25 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific VPS25 antibody is suitable for use in Western blot/Immunohistochemistry/ELISA applications with human and rat samples.
- Reactivity
- Human, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the VPS25 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human liver lysate with VPS25 antibody at 0.5ug/ml. Predicted molecular weight: ~21kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of rat kidney lysate with VPS25 antibody at 0.5ug/ml. Predicted molecular weight: ~21kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human liver lysate with VPS25 antibody at 0.5ug/ml. Predicted molecular weight: ~21kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human kidney with VPS25 antibody at 10ug/ml. HIER: microwaved with pH9 Tris/EDTA buffer, HRP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human kidney tissue with (left) and without (right) VPS25 antibody at 6ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer.
