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- Antibody Data
- Antigen structure
- References [37]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 13398-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#13398-1-AP, RRID:AB_2113965
- Product name
- GPR161 antibody
- Antibody type
- Polyclonal
- Description
- GPR161 antibody (Cat. #13398-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Rgl-exomiR-7972, a novel plant exosomal microRNA derived from fresh Rehmanniae Radix, ameliorated lipopolysaccharide-induced acute lung injury and gut dysbiosis.
Certain heterozygous variants in the kinase domain of the serine/threonine kinase NEK8 can cause an autosomal dominant form of polycystic kidney disease.
Multiple interactions of the dynein-2 complex with the IFT-B complex are required for effective intraflagellar transport.
Dynein-2-driven intraciliary retrograde trafficking indirectly requires multiple interactions of IFT54 in the IFT-B complex with the dynein-2 complex.
Ubiquitylation of BBSome is required for ciliary assembly and signaling.
The ancestral ESCRT protein TOM1L2 selects ubiquitinated cargoes for retrieval from cilia.
Systematic analysis of cilia characteristics and Hedgehog signaling in five immortal cell lines.
KIF24 depletion induces clustering of supernumerary centrosomes in PDAC cells.
The ciliary gene INPP5E confers dorsal telencephalic identity to human cortical organoids by negatively regulating Sonic hedgehog signaling.
ARL3 and ARL13B GTPases participate in distinct steps of INPP5E targeting to the ciliary membrane.
Vertebrate cells differentially interpret ciliary and extraciliary cAMP.
Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.
Interaction of INPP5E with ARL13B is essential for its ciliary membrane retention but dispensable for its ciliary entry.
Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.
Cooperation of the IFT-A complex with the IFT-B complex is required for ciliary retrograde protein trafficking and GPCR import.
Bisdemethoxycurcumin Promotes Apoptosis and Inhibits the Epithelial-Mesenchymal Transition through the Inhibition of the G-Protein-Coupled Receptor 161/Mammalian Target of Rapamycin Signaling Pathway in Triple Negative Breast Cancer Cells.
Differential Roles of Tubby Family Proteins in Ciliary Formation and Trafficking.
FBF1 deficiency promotes beiging and healthy expansion of white adipose tissue.
Enterotoxigenic Escherichia coli infection promotes enteric defensin expression via FOXO6-METTL3-m(6)A-GPR161 signalling axis.
Roles of TOG and jelly-roll domains of centrosomal protein CEP104 in its functions in cilium elongation and Hedgehog signaling.
Primary cilium remodeling mediates a cell signaling switch in differentiating neurons.
Ubiquitin chains earmark GPCRs for BBSome-mediated removal from cilia.
Requirement of IFT-B-BBSome complex interaction in export of GPR161 from cilia.
Cannabinoids Exacerbate Alcohol Teratogenesis by a CB1-Hedgehog Interaction.
C11ORF74 interacts with the IFT-A complex and participates in ciliary BBSome localization.
TULP3 is required for localization of membrane-associated proteins ARL13B and INPP5E to primary cilia.
RABL2 positively controls localization of GPCRs in mammalian primary cilia.
Interaction of WDR60 intermediate chain with TCTEX1D2 light chain of the dynein-2 complex is crucial for ciliary protein trafficking.
Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis.
Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling.
Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis.
The Meckel syndrome- associated protein MKS1 functionally interacts with components of the BBSome and IFT complexes to mediate ciliary trafficking and hedgehog signaling.
A compartmentalized phosphoinositide signaling axis at cilia is regulated by INPP5E to maintain cilia and promote Sonic Hedgehog medulloblastoma.
INPP5E regulates phosphoinositide-dependent cilia transition zone function.
Regulation of ciliary retrograde protein trafficking by the Joubert syndrome proteins ARL13B and INPP5E.
Conserved Genetic Interactions between Ciliopathy Complexes Cooperatively Support Ciliogenesis and Ciliary Signaling.
Phosphoinositides Regulate Ciliary Protein Trafficking to Modulate Hedgehog Signaling.
Qiu FS, Wang JF, Guo MY, Li XJ, Shi CY, Wu F, Zhang HH, Ying HZ, Yu CH
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Sep;165:115007
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Sep;165:115007
Certain heterozygous variants in the kinase domain of the serine/threonine kinase NEK8 can cause an autosomal dominant form of polycystic kidney disease.
Claus LR, Chen C, Stallworth J, Turner JL, Slaats GG, Hawks AL, Mabillard H, Senum SR, Srikanth S, Flanagan-Steet H, Louie RJ, Silver J, Lerner-Ellis J, Morel C, Mighton C, Sleutels F, van Slegtenhorst M, van Ham T, Brooks AS, Dorresteijn EM, Barakat TS, Dahan K, Demoulin N, Goffin EJ, Olinger E, Genomics England Research Consortium, Larsen M, Hertz JM, Lilien MR, Obeidová L, Seeman T, Stone HK, Kerecuk L, Gurgu M, Yousef Yengej FA, Ammerlaan CME, Rookmaaker MB, Hanna C, Rogers RC, Duran K, Peters E, Sayer JA, van Haaften G, Harris PC, Ling K, Mason JM, van Eerde AM, Steet R
Kidney international 2023 Nov;104(5):995-1007
Kidney international 2023 Nov;104(5):995-1007
Multiple interactions of the dynein-2 complex with the IFT-B complex are required for effective intraflagellar transport.
Hiyamizu S, Qiu H, Vuolo L, Stevenson NL, Shak C, Heesom KJ, Hamada Y, Tsurumi Y, Chiba S, Katoh Y, Stephens DJ, Nakayama K
Journal of cell science 2023 Mar 1;136(5)
Journal of cell science 2023 Mar 1;136(5)
Dynein-2-driven intraciliary retrograde trafficking indirectly requires multiple interactions of IFT54 in the IFT-B complex with the dynein-2 complex.
Hiyamizu S, Qiu H, Tsurumi Y, Hamada Y, Katoh Y, Nakayama K
Biology open 2023 Jul 15;12(7)
Biology open 2023 Jul 15;12(7)
Ubiquitylation of BBSome is required for ciliary assembly and signaling.
Chiuso F, Delle Donne R, Giamundo G, Rinaldi L, Borzacchiello D, Moraca F, Intartaglia D, Iannucci R, Senatore E, Lignitto L, Garbi C, Conflitti P, Catalanotti B, Conte I, Feliciello A
EMBO reports 2023 Apr 5;24(4):e55571
EMBO reports 2023 Apr 5;24(4):e55571
The ancestral ESCRT protein TOM1L2 selects ubiquitinated cargoes for retrieval from cilia.
Shinde SR, Mick DU, Aoki E, Rodrigues RB, Gygi SP, Nachury MV
Developmental cell 2023 Apr 24;58(8):677-693.e9
Developmental cell 2023 Apr 24;58(8):677-693.e9
Systematic analysis of cilia characteristics and Hedgehog signaling in five immortal cell lines.
Gómez AE, Christman AK, Van De Weghe JC, Finn M, Doherty D
PloS one 2022;17(12):e0266433
PloS one 2022;17(12):e0266433
KIF24 depletion induces clustering of supernumerary centrosomes in PDAC cells.
Mashima Y, Nohira H, Sugihara H, Dynlacht BD, Kobayashi T, Itoh H
Life science alliance 2022 Nov;5(11)
Life science alliance 2022 Nov;5(11)
The ciliary gene INPP5E confers dorsal telencephalic identity to human cortical organoids by negatively regulating Sonic hedgehog signaling.
Schembs L, Willems A, Hasenpusch-Theil K, Cooper JD, Whiting K, Burr K, Bøstrand SMK, Selvaraj BT, Chandran S, Theil T
Cell reports 2022 May 17;39(7):110811
Cell reports 2022 May 17;39(7):110811
ARL3 and ARL13B GTPases participate in distinct steps of INPP5E targeting to the ciliary membrane.
Fujisawa S, Qiu H, Nozaki S, Chiba S, Katoh Y, Nakayama K
Biology open 2021 Sep 15;10(9)
Biology open 2021 Sep 15;10(9)
Vertebrate cells differentially interpret ciliary and extraciliary cAMP.
Truong ME, Bilekova S, Choksi SP, Li W, Bugaj LJ, Xu K, Reiter JF
Cell 2021 May 27;184(11):2911-2926.e18
Cell 2021 May 27;184(11):2911-2926.e18
Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.
Chen C, Xu Q, Zhang Y, Davies BA, Huang Y, Katzmann DJ, Harris PC, Hu J, Ling K
Science advances 2021 Jun;7(26)
Science advances 2021 Jun;7(26)
Interaction of INPP5E with ARL13B is essential for its ciliary membrane retention but dispensable for its ciliary entry.
Qiu H, Fujisawa S, Nozaki S, Katoh Y, Nakayama K
Biology open 2021 Jan 25;10(1)
Biology open 2021 Jan 25;10(1)
Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.
Duan S, Li H, Zhang Y, Yang S, Chen Y, Qiu B, Huang C, Wang J, Li J, Zhu X, Yan X
The EMBO journal 2021 Jan 15;40(2):e105499
The EMBO journal 2021 Jan 15;40(2):e105499
Cooperation of the IFT-A complex with the IFT-B complex is required for ciliary retrograde protein trafficking and GPCR import.
Kobayashi T, Ishida Y, Hirano T, Katoh Y, Nakayama K
Molecular biology of the cell 2021 Jan 1;32(1):45-56
Molecular biology of the cell 2021 Jan 1;32(1):45-56
Bisdemethoxycurcumin Promotes Apoptosis and Inhibits the Epithelial-Mesenchymal Transition through the Inhibition of the G-Protein-Coupled Receptor 161/Mammalian Target of Rapamycin Signaling Pathway in Triple Negative Breast Cancer Cells.
Chou CH, Wang HK, Lin YC, Tsai DH, Lu MT, Ho CT, Hseu YC, Yang HL, Way TD
Journal of agricultural and food chemistry 2021 Dec 8;69(48):14557-14567
Journal of agricultural and food chemistry 2021 Dec 8;69(48):14557-14567
Differential Roles of Tubby Family Proteins in Ciliary Formation and Trafficking.
Hong JJ, Kim KE, Park SY, Bok J, Seo JT, Moon SJ
Molecules and cells 2021 Aug 31;44(8):591-601
Molecules and cells 2021 Aug 31;44(8):591-601
FBF1 deficiency promotes beiging and healthy expansion of white adipose tissue.
Zhang Y, Hao J, Tarrago MG, Warner GM, Giorgadze N, Wei Q, Huang Y, He K, Chen C, Peclat TR, White TA, Ling K, Tchkonia T, Kirkland JL, Chini EN, Hu J
Cell reports 2021 Aug 3;36(5):109481
Cell reports 2021 Aug 3;36(5):109481
Enterotoxigenic Escherichia coli infection promotes enteric defensin expression via FOXO6-METTL3-m(6)A-GPR161 signalling axis.
Zong X, Wang H, Xiao X, Zhang Y, Hu Y, Wang F, Wang Y, Lu Z
RNA biology 2021 Apr;18(4):576-586
RNA biology 2021 Apr;18(4):576-586
Roles of TOG and jelly-roll domains of centrosomal protein CEP104 in its functions in cilium elongation and Hedgehog signaling.
Yamazoe T, Nagai T, Umeda S, Sugaya Y, Mizuno K
The Journal of biological chemistry 2020 Oct 23;295(43):14723-14736
The Journal of biological chemistry 2020 Oct 23;295(43):14723-14736
Primary cilium remodeling mediates a cell signaling switch in differentiating neurons.
Toro-Tapia G, Das RM
Science advances 2020 May;6(21):eabb0601
Science advances 2020 May;6(21):eabb0601
Ubiquitin chains earmark GPCRs for BBSome-mediated removal from cilia.
Shinde SR, Nager AR, Nachury MV
The Journal of cell biology 2020 Dec 7;219(12)
The Journal of cell biology 2020 Dec 7;219(12)
Requirement of IFT-B-BBSome complex interaction in export of GPR161 from cilia.
Nozaki S, Castro Araya RF, Katoh Y, Nakayama K
Biology open 2019 Sep 17;8(9)
Biology open 2019 Sep 17;8(9)
Cannabinoids Exacerbate Alcohol Teratogenesis by a CB1-Hedgehog Interaction.
Fish EW, Murdaugh LB, Zhang C, Boschen KE, Boa-Amponsem O, Mendoza-Romero HN, Tarpley M, Chdid L, Mukhopadhyay S, Cole GJ, Williams KP, Parnell SE
Scientific reports 2019 Nov 5;9(1):16057
Scientific reports 2019 Nov 5;9(1):16057
C11ORF74 interacts with the IFT-A complex and participates in ciliary BBSome localization.
Takahara M, Kunii M, Nakamura K, Harada A, Hirano T, Katoh Y, Nakayama K
Journal of biochemistry 2019 Mar 1;165(3):257-267
Journal of biochemistry 2019 Mar 1;165(3):257-267
TULP3 is required for localization of membrane-associated proteins ARL13B and INPP5E to primary cilia.
Han S, Miyoshi K, Shikada S, Amano G, Wang Y, Yoshimura T, Katayama T
Biochemical and biophysical research communications 2019 Jan 29;509(1):227-234
Biochemical and biophysical research communications 2019 Jan 29;509(1):227-234
RABL2 positively controls localization of GPCRs in mammalian primary cilia.
Dateyama I, Sugihara Y, Chiba S, Ota R, Nakagawa R, Kobayashi T, Itoh H
Journal of cell science 2019 Jan 16;132(2)
Journal of cell science 2019 Jan 16;132(2)
Interaction of WDR60 intermediate chain with TCTEX1D2 light chain of the dynein-2 complex is crucial for ciliary protein trafficking.
Hamada Y, Tsurumi Y, Nozaki S, Katoh Y, Nakayama K
Molecular biology of the cell 2018 Jul 1;29(13):1628-1639
Molecular biology of the cell 2018 Jul 1;29(13):1628-1639
Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis.
Takahara M, Katoh Y, Nakamura K, Hirano T, Sugawa M, Tsurumi Y, Nakayama K
Human molecular genetics 2018 Feb 1;27(3):516-528
Human molecular genetics 2018 Feb 1;27(3):516-528
Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling.
Hong SR, Wang CL, Huang YS, Chang YC, Chang YC, Pusapati GV, Lin CY, Hsu N, Cheng HC, Chiang YC, Huang WE, Shaner NC, Rohatgi R, Inoue T, Lin YC
Nature communications 2018 Apr 30;9(1):1732
Nature communications 2018 Apr 30;9(1):1732
Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis.
Takei R, Katoh Y, Nakayama K
Biology open 2018 Apr 30;7(5)
Biology open 2018 Apr 30;7(5)
The Meckel syndrome- associated protein MKS1 functionally interacts with components of the BBSome and IFT complexes to mediate ciliary trafficking and hedgehog signaling.
Goetz SC, Bangs F, Barrington CL, Katsanis N, Anderson KV
PloS one 2017;12(3):e0173399
PloS one 2017;12(3):e0173399
A compartmentalized phosphoinositide signaling axis at cilia is regulated by INPP5E to maintain cilia and promote Sonic Hedgehog medulloblastoma.
Conduit SE, Ramaswamy V, Remke M, Watkins DN, Wainwright BJ, Taylor MD, Mitchell CA, Dyson JM
Oncogene 2017 Oct 26;36(43):5969-5984
Oncogene 2017 Oct 26;36(43):5969-5984
INPP5E regulates phosphoinositide-dependent cilia transition zone function.
Dyson JM, Conduit SE, Feeney SJ, Hakim S, DiTommaso T, Fulcher AJ, Sriratana A, Ramm G, Horan KA, Gurung R, Wicking C, Smyth I, Mitchell CA
The Journal of cell biology 2017 Jan 2;216(1):247-263
The Journal of cell biology 2017 Jan 2;216(1):247-263
Regulation of ciliary retrograde protein trafficking by the Joubert syndrome proteins ARL13B and INPP5E.
Nozaki S, Katoh Y, Terada M, Michisaka S, Funabashi T, Takahashi S, Kontani K, Nakayama K
Journal of cell science 2017 Feb 1;130(3):563-576
Journal of cell science 2017 Feb 1;130(3):563-576
Conserved Genetic Interactions between Ciliopathy Complexes Cooperatively Support Ciliogenesis and Ciliary Signaling.
Yee LE, Garcia-Gonzalo FR, Bowie RV, Li C, Kennedy JK, Ashrafi K, Blacque OE, Leroux MR, Reiter JF
PLoS genetics 2015 Nov;11(11):e1005627
PLoS genetics 2015 Nov;11(11):e1005627
Phosphoinositides Regulate Ciliary Protein Trafficking to Modulate Hedgehog Signaling.
Garcia-Gonzalo FR, Phua SC, Roberson EC, Garcia G 3rd, Abedin M, Schurmans S, Inoue T, Reiter JF
Developmental cell 2015 Aug 24;34(4):400-409
Developmental cell 2015 Aug 24;34(4):400-409
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 13398-1-AP(GPR161 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human placenta using 13398-1-AP(GPR161 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The GPR161 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human GPR161. This antibody recognizes human,mouse,rat antigen. The GPR161 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
