Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA3-065 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Endothelin A Receptor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- PA3-065 detects Endothelin Receptor A from human, mouse, and rat samples. PA3-065 has been successfully used in Western blot, immunocytochemistry, and immunohistochemistry procedures. By western blot, this antibody detects a 46kDa protein corresponding to mouse Endothelin Receptor A.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Endothelin-1 induces changes in the expression levels of steroidogenic enzymes and increases androgen receptor and testosterone production in the PC3 prostate cancer cell line.
Peroxisome proliferator-activated receptor gamma blunts endothelin-1-mediated contraction of the uterine artery in a murine model of high-altitude pregnancy.
Reassessment of endothelin receptor A expression in normal and neoplastic human tissues using the novel rabbit monoclonal antibody UMB-8.
Transforming growth factor-β regulates endothelin-1 signaling in the newborn mouse lung during hypoxia exposure.
Torres MJ, López-Moncada F, Herrera D, Indo S, Lefian A, Llanos P, Tapia J, Castellón EA, Contreras HR
Oncology reports 2021 Aug;46(2)
Oncology reports 2021 Aug;46(2)
Peroxisome proliferator-activated receptor gamma blunts endothelin-1-mediated contraction of the uterine artery in a murine model of high-altitude pregnancy.
Lane SL, Doyle AS, Bales ES, Houck JA, Lorca RA, Moore LG, Julian CG
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Mar;34(3):4283-4292
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Mar;34(3):4283-4292
Reassessment of endothelin receptor A expression in normal and neoplastic human tissues using the novel rabbit monoclonal antibody UMB-8.
Lupp A, Mann A, Heeb A, Kaemmerer D, Sänger J, Evert M, Evert K, Mawrin C, Schulz S
Peptides 2015 Apr;66:19-25
Peptides 2015 Apr;66:19-25
Transforming growth factor-β regulates endothelin-1 signaling in the newborn mouse lung during hypoxia exposure.
Olave N, Nicola T, Zhang W, Bulger A, James M, Oparil S, Chen YF, Ambalavanan N
American journal of physiology. Lung cellular and molecular physiology 2012 May 1;302(9):L857-65
American journal of physiology. Lung cellular and molecular physiology 2012 May 1;302(9):L857-65
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Endothelin A Receptor in mouse heart using a polyclonal antibody (Product # PA3-065).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Endothelin A Receptor Polyclonal Antibody (Product # PA3-065) and a 50kDa band corresponding to Endothelin A Receptor was observed across tissues tested. Tissue extracts (40 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2), Mouse Cerebellum (Lane 3), Rat Cerebellum (Lane 4), Mouse Lung (Lane 5), Rat Lung (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Endothelin A Receptor in ETA-transfected cells using a polyclonal antibody (Product # PA3-065).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Endothelin A Receptor in ETA-transfected cells using a polyclonal antibody (Product # PA3-065).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of ETA in mouse dorsal root ganglia
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of ETA receptors in the atrium of mouse heart.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on acetone-fixed frozen mouse skin tissue sections. Tissues were fixed for 10 minutes, blocked in 3% fish gelatin for 10-20 minutes, and probed with an Isotype Control Antibody (panel A), and Endothelin Receptor A polyclonal antibody (Product # PA3-065, panel B), or an Endothelin Receptor B polyclonal antibody (Product # PA3-066, panel C) all at dilutions of 1:800 overnight at 4C. Detection was performed using a PE-conjugated anti-rabbit IgG secondary antibody at a dilution of 1:800 (red). Nuclei were stained with DAPI and tissues were visualized by fluorescence microscopy. Data courtesy of the Innovators Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on acetone-fixed frozen mouse skin tissue sections. Tissues were fixed for 10 minutes, blocked in 3% fish gelatin for 10-20 minutes, and probed with an Isotype Control Antibody (panel A), and Endothelin Receptor A polyclonal antibody (Product # PA3-065, panel B), or an Endothelin Receptor B polyclonal antibody (Product # PA3-066, panel C) all at dilutions of 1:800 overnight at 4C. Detection was performed using a PE-conjugated anti-rabbit IgG secondary antibody at a dilution of 1:800 (red). Nuclei were stained with DAPI and tissues were visualized by fluorescence microscopy. Data courtesy of the Innovators Program.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2. ET A R and ET B R expression in prostate cancer cells. (A) ET A R mRNA expression normalized to Pumilio compared with PC3 cells. (B) ET A R protein expression in PC3 and LNCaP cells. Quantification was normalized to beta-actin and PC3 cells. (C) ET B R mRNA expression normalized to Pumilio compared with LNCaP cells. (D) ET B R protein expression in PC3 and LNCaP cells normalized to Pumilio compared with LNCaP cells. Immunofluorescence analysis of (E) ET A R and (F) ET B R in PC3 and LNCaP cells. (G) Negative controls. Data are presented as the mean +- SD (n=3 independent experiments) of arbitrary units. *P