PA3-065

antibody from Invitrogen Antibodies

Targeting: EDNRA ET-A, ETA-R, hET-AR

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA3-065

Provider

Invitrogen Antibodies

Product name

Endothelin A Receptor Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

PA3-065 detects Endothelin Receptor A from human, mouse, and rat samples. PA3-065 has been successfully used in Western blot, immunocytochemistry, and immunohistochemistry procedures. By western blot, this antibody detects a 46kDa protein corresponding to mouse Endothelin Receptor A.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Endothelin-1 induces changes in the expression levels of steroidogenic enzymes and increases androgen receptor and testosterone production in the PC3 prostate cancer cell line.

Torres MJ, López-Moncada F, Herrera D, Indo S, Lefian A, Llanos P, Tapia J, Castellón EA, Contreras HR
Oncology reports 2021 Aug;46(2)

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Peroxisome proliferator-activated receptor gamma blunts endothelin-1-mediated contraction of the uterine artery in a murine model of high-altitude pregnancy.

Lane SL, Doyle AS, Bales ES, Houck JA, Lorca RA, Moore LG, Julian CG
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Mar;34(3):4283-4292

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Reassessment of endothelin receptor A expression in normal and neoplastic human tissues using the novel rabbit monoclonal antibody UMB-8.

Lupp A, Mann A, Heeb A, Kaemmerer D, Sänger J, Evert M, Evert K, Mawrin C, Schulz S
Peptides 2015 Apr;66:19-25

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Transforming growth factor-β regulates endothelin-1 signaling in the newborn mouse lung during hypoxia exposure.

Olave N, Nicola T, Zhang W, Bulger A, James M, Oparil S, Chen YF, Ambalavanan N
American journal of physiology. Lung cellular and molecular physiology 2012 May 1;302(9):L857-65

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Endothelin A Receptor in ETA-transfected cells using a polyclonal antibody (Product # PA3-065).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Endothelin A Receptor in ETA-transfected cells using a polyclonal antibody (Product # PA3-065).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2. ET A R and ET B R expression in prostate cancer cells. (A) ET A R mRNA expression normalized to Pumilio compared with PC3 cells. (B) ET A R protein expression in PC3 and LNCaP cells. Quantification was normalized to beta-actin and PC3 cells. (C) ET B R mRNA expression normalized to Pumilio compared with LNCaP cells. (D) ET B R protein expression in PC3 and LNCaP cells normalized to Pumilio compared with LNCaP cells. Immunofluorescence analysis of (E) ET A R and (F) ET B R in PC3 and LNCaP cells. (G) Negative controls. Data are presented as the mean +- SD (n=3 independent experiments) of arbitrary units. *P