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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-13432 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Endothelin A Receptor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using an EDNRA polyclonal antibody (Product # PA5-13432) in CEM cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using an EDNRA polyclonal antibody (Product # PA5-13432) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CEM cells using an EDNRA polyclonal antibody (Product # PA5-13432) (green), compared to a negative control cell (blue) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of EDNRA on mouse bone marrow cells. Cells were harvested, fixed with 2% formalin for 15 minutes at room temperature, washed with 2% BSA in PBS, and incubated with an EDNRA polyclonal antibody (Product # PA5-13432) at dilutions of 1:10 (orange histogram), 1:50 (blue histogram), and 1:100 (green histogram), or a rabbit IgG isotype control (red histogram) for 30 minutes at room temperature. Following the primary antibody incubation, cells were washed, and then incubated with an Alexa Fluor 555-conjugated anti-rabbit IgG secondary antibody (Product # A-31572) for 30 minutes at room temperature. Cells were washed again, and the same number of events per sample was acquired on a flow cytometer. Data courtesy of the Innovators Program.
