PA5-30589

antibody from Invitrogen Antibodies

Targeting: EDNRA ET-A, ETA-R, hET-AR

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30589

Provider

Invitrogen Antibodies

Product name

Endothelin A Receptor Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: U87-MG, GL261.

Concentration

0.78 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Endothelin A Receptor using 30 µg of U87-MG lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Endothelin A Receptor polyclonal antibody (Product # PA5-30589) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Endothelin A Receptor using 30 µg of GL261 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Endothelin A Receptor polyclonal antibody (Product # PA5-30589) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Endothelin A Receptor was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Endothelin A Receptor Polyclonal Antibody (Product # PA5-30589) at a dilution of 1:0. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Endothelin A Receptor Polyclonal Antibody (Product # PA5-30589). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Endothelin A Receptor Polyclonal Antibody (Product # PA5-30589) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Endothelin A Receptor (Product # PA5-30589) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.