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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- PA5-30589 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Endothelin A Receptor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: U87-MG, GL261. Predicted reactivity: Rhesus Monkey (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.78 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Endothelin A Receptor using 30 µg of U87-MG lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Endothelin A Receptor polyclonal antibody (Product # PA5-30589) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Endothelin A Receptor using 30 µg of GL261 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Endothelin A Receptor polyclonal antibody (Product # PA5-30589) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Endothelin A Receptor was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Endothelin A Receptor Polyclonal Antibody (Product # PA5-30589) at a dilution of 1:0. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Endothelin A Receptor Polyclonal Antibody (Product # PA5-30589). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Endothelin A Receptor Polyclonal Antibody (Product # PA5-30589) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Endothelin A Receptor (Product # PA5-30589) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
