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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- TA500694 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500694, RRID:AB_11140725
- Product name
- Anti-PSMA7 mouse monoclonal antibody, clone OTI8F9 (formerly 8F9)
- Antibody type
- Monoclonal
- Description
- Anti-PSMA7 mouse monoclonal antibody, clone OTI8F9 (formerly 8F9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PSMA7
- Isotype
- IgG
- Antibody clone number
- OTI8F9
- Vial size
- 100 µl
- Concentration
- 0.68 mg/ml
Submitted references Novel autoantibodies against the proteasome subunit PSMA7 in amyotrophic lateral sclerosis.
Sugimoto K, Hiwasa T, Shibuya K, Hirano S, Beppu M, Isose S, Arai K, Takiguchi M, Kuwabara S, Mori M
Journal of neuroimmunology 2018 Dec 15;325:54-60
Journal of neuroimmunology 2018 Dec 15;325:54-60
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA7 (RC201169, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA7.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-PSMA7 mouse monoclonal antibody (TA500694) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMA7(RC201169).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PSMA7mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500694, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-PSMA7mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500694, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-PSMA7mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500694, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-PSMA7 antibody(TA500694),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Validation comment
- FC
