Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB4165 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Collagen XXIII alpha 1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Collagen XXIII alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50% cross-reactivity with recombinant human Collagen XXV alpha 1 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_775736
- Isotype
- IgG
- Antibody clone number
- 468642
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A role for collagen XXIII in cancer cell adhesion, anchorage-independence and metastasis.
Spivey KA, Chung I, Banyard J, Adini I, Feldman HA, Zetter BR
Oncogene 2012 May 3;31(18):2362-72
Oncogene 2012 May 3;31(18):2362-72
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Collagen XXIII alpha 1 by Western Blot. Western blot shows lysates of NCI-H460 human large cell lung carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human Collagen XXIII alpha 1 Monoclonal Antibody (Catalog # MAB4165) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Collagen XXIII alpha 1 at approximately 75 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Collagen XXIII alpha 1 in Human Adenocarcinoma. Collagen XXIII alpha 1 was detected in immersion fixed paraffin-embedded sections of human adenocarcinoma using Human Collagen XXIII alpha 1 Monoclonal Antibody (Catalog # MAB4165) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.