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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-85082 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-GAPDH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Cat (100%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%). Positive Control: A431, H1299, HeLa, HepG2, Neuro2A, BCL-1, Raw264.7, SH-SY5Y. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GAPDH in A) Neuro2A whole cell lysate, B) BCL-1 whole cell lysate, C) Raw264.7 whole cell lysate/extract using GAPDH polyclonal antibody (Product # PA5-85082) using 30 µg of sample at a dilution of 1:2000. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GAPDH in A) Hep G2 cells using GAPDH polyclonal antibody (Product # PA5-85082) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 10% SDS PAGE.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of GAPDH in HeLa cells using GAPDH polyclonal antibody (Product # PA5-85082) at a dilution of 1:100.
