703703

antibody from Invitrogen Antibodies

Targeting: ACKR1 CCBP1, CD234, DARC, Dfy, FY, GPD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

703703

Provider

Invitrogen Antibodies

Product name

DARC Recombinant Rabbit Monoclonal Antibody (10H52L38)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

10H52L38

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of K-562 (Lane 1) MDA-MB-231 (Lane 2), MCF-7 (Lane 3) and MCF 10A (Lane 4). The blot was probed wit Anti-DARC Recombinant Rabbit Monoclonal Antibody (Product # 703703, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). A ~34 kDa band corresponding to DARC was observed in the relevant cell lines tested.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, MCF-7 cells were fixed and permeabilized for detection of endogenous DARC using Anti-DARC Recombinant Rabbit Monoclonal Antibody (Product # 703703, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # 703404, 1:2000). Panel a) shows representative cells that were stained for detection and localization of DARC protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating membrane localization of DARC. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.