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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA328639 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-Melanocortin Receptor 4 (extracellular)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-Melanocortin Receptor 4 (extracellular)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MC4R
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain lysates: 1. Anti-Melanocortin Receptor 4 (extracellular) antibody, (1:400). 2. Anti-Melanocortin Receptor 4 (extracellular) antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of mouse brain lysates: 1. Anti-Melanocortin Receptor 4 (extracellular) antibody, (1:400). 2. Anti-Melanocortin Receptor 4 (extracellular) antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of MC4R in rat pituitary cell line. Immunocytochemical staining of live intact GH3 cells with Anti-Melanocortin Receptor 4 (extracellular) antibody, (1:50). A. MC4R staining was observed, followed by Alexa-555-conjugated goat anti-rabbit secondary antibody (red staining). Hoechst 33342 (blue) is used to visualize the nuclei. B. Live view of the same field as A.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of MC4R in mouse brain. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Melanocortin Receptor 4 (extracellular) antibody, (1:100). MC4R (green fluorescence) is expressed in the mouse hypothalamus in axonal processes (arrows). Hoechst 33342 is used as the counterstain (blue).
- Validation comment
- IHC
