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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [3]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-95277 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP1B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Human CYP1B1 shares 85.4% and 84.5% amino acid (aa) sequence identity with mouse and rat CYP1B1, respectively. Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Attenuation of Polycyclic Aromatic Hydrocarbon (PAH)-Mediated Pulmonary DNA Adducts and Cytochrome P450 (CYP)1B1 by Dietary Antioxidants, Omega-3 Fatty Acids, in Mice.
Zhou G, Jiang W, Xia G, Wang L, Richardson M, Chu C, Moorthy B
Antioxidants (Basel, Switzerland) 2022 Jan 5;11(1)
Antioxidants (Basel, Switzerland) 2022 Jan 5;11(1)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CYP1B1 in paraffin-embedded rat kidney tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with CYP1B1 polyclonal antibody (Product # PA5-95277) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CYP1B1 in paraffin-embedded human liver cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with CYP1B1 polyclonal antibody (Product # PA5-95277) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CYP1B1 in paraffin-embedded mouse kidney tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with CYP1B1 polyclonal antibody (Product # PA5-95277) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CYP1B1 in SiHa cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with CYP1B1 polyclonal antibody (Product # PA5-95277) at a dilution of 1 µg (per 1x10^6 cells), followed by 488 conjugated goat anti-rabbit IgG (30 min at 20°C) using a 5-10 µg (per 1x10^6 cells) dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Proteins isolated from liver and lung of mice, fed with CO, FO, EPA, DHA, EPA/DHA and treated with BP, were probed with Cyp1A1, 1B1 or 1A2 (liver only) antibodies by Western blot. FO, DHA and EPA + DHA significantly inhibited Cyp1b1 compared to CO group. Hepatic Cyp1A2 induced by BP treatment was also inhibited by FO, EPA, and DHA. *, p < 0.05, **, p < 0.01, ( n = 5).
