Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-23139 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP1B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CYP1B1in human brain lysate in the 1) absence and 2) presence of immunizing peptide using a Cytochrome P450 (CYP1B1) polyclonal antibody (Product # PA5-23139) at1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CYP1B1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide. Samples were incubated in CYP1B1 polyclonal antibody (Product # PA5-23139) using a dilution of 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CYP1B1 Polyclonal Antibody(Product # PA5-23139) and a 61kDa band corresponding to CYP1B1 was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), A549 (Lane 2), PANC-1 (Lane 3), SK-O-V3 (Lane 4), DU 145 (Lane 5), K-562 (Lane 6), BeWo (Lane 7), Hep G2 (Lane 8), Daudi (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CYP1B1 in human brain tissue. Samples were incubated in CYP1B1 polyclonal antibody (Product # PA5-23139) using a dilution of 5 µg/mL. Isotype control (top left) and this antibody (bottom left, right).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CYP1B1 in human adrenal. Samples were incubated in CYP1B1 polyclonal antibody (Product # PA5-23139) using a dilution of 10 µg/mL.