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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
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- Product number
- PA5-28040 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP1B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, mouse lung. Predicted reactivity: Mouse (84%), Rat (84%), Dog (82%), Rhesus Monkey (94%), Bovine (82%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.63 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Fumonisin B₁ modulates expression of human cytochrome P450 1b1 in human hepatoma (Hepg2) cells by repressing Mir-27b.
Chuturgoon AA, Phulukdaree A, Moodley D
Toxicology letters 2014 May 16;227(1):50-5
Toxicology letters 2014 May 16;227(1):50-5
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cytochrome P450 1B1 using 30 µg 293T whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Cytochrome P450 1B1 polyclonal antibody (Product # PA5-28040) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CYP1B1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CYP1B1 Polyclonal Antibody (Product # PA5-28040) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CYP1B1 Polyclonal Antibody (Product # PA5-28040). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP1B1 Polyclonal Antibody (Product # PA5-28040) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CYP1B1 Polyclonal Antibody detects CYP1B1 protein by western blot analysis. A. 50 µg mouse lung lysate/extract.7.5% SDS-PAGE. CYP1B1 Polyclonal Antibody (Product # PA5-28040) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cytochrome P450 1B1 in methanol-fixed HeLa cells using a Cytochrome P450 1B1 polyclonal antibody (Product # PA5-28040) at a 1:200 dilution.
