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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- 13498-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#13498-1-AP, RRID:AB_2183747
- Product name
- RUFY1 antibody
- Antibody type
- Polyclonal
- Description
- RUFY1 antibody (Cat. #13498-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Mouse oocytes sequester aggregated proteins in degradative super-organelles.
Podocalyxin-like protein promotes gastric cancer progression through interacting with RUN and FYVE domain containing 1 protein.
Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5.
COMMD1 is linked to the WASH complex and regulates endosomal trafficking of the copper transporter ATP7A.
Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks.
Zaffagnini G, Cheng S, Salzer MC, Pernaute B, Duran JM, Irimia M, Schuh M, Böke E
Cell 2024 Feb 29;187(5):1109-1126.e21
Cell 2024 Feb 29;187(5):1109-1126.e21
Podocalyxin-like protein promotes gastric cancer progression through interacting with RUN and FYVE domain containing 1 protein.
Zhi Q, Chen H, Liu F, Han Y, Wan D, Xu Z, Kuang Y, Zhou J
Cancer science 2019 Jan;110(1):118-134
Cancer science 2019 Jan;110(1):118-134
Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5.
Shi M, Chen B, Mahajan D, Boh BK, Zhou Y, Dutta B, Tie HC, Sze SK, Wu G, Lu L
Nature communications 2018 Nov 26;9(1):4987
Nature communications 2018 Nov 26;9(1):4987
COMMD1 is linked to the WASH complex and regulates endosomal trafficking of the copper transporter ATP7A.
Phillips-Krawczak CA, Singla A, Starokadomskyy P, Deng Z, Osborne DG, Li H, Dick CJ, Gomez TS, Koenecke M, Zhang JS, Dai H, Sifuentes-Dominguez LF, Geng LN, Kaufmann SH, Hein MY, Wallis M, McGaughran J, Gecz J, Sluis Bv, Billadeau DD, Burstein E
Molecular biology of the cell 2015 Jan 1;26(1):91-103
Molecular biology of the cell 2015 Jan 1;26(1):91-103
Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks.
Gomez TS, Gorman JA, de Narvajas AA, Koenig AO, Billadeau DD
Molecular biology of the cell 2012 Aug;23(16):3215-28
Molecular biology of the cell 2012 Aug;23(16):3215-28
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 13498-1-AP(RUFY1 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using RUFY1 antibody 13498-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP result of anti-RUFY1(13498-1-AP for IP and Detection).
- Sample type
- cell line
