13498-1-AP

antibody from Proteintech Group

Targeting: RUFY1 FLJ22251, RABIP4, ZFYVE12

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

13498-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#13498-1-AP, RRID:AB_2183747

Product name

RUFY1 antibody

Antibody type

Polyclonal

Description

RUFY1 antibody (Cat. #13498-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

The maternal PADI6-UHRF1-UBE2D complex regulates ubiquitination during oocyte maturation and embryogenesis.

Li J, Lu Y, Xia Z, Chi P, Qi Q, Liu S, Ju S, Li J, Zhang Z, Han Z, Liu Q, Meng W, Chen J, Wang X, Guo L, Li L, Huang W, Dai L, Han J, Gao S, Deng D
Nature structural & molecular biology 2026 Mar;33(3):512-524

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CCDC41 Drives Oocyte Meiotic Progression by Promoting Rab11a/Rab7-Positive Vesicle Fusion with Target Membranes.

Tian Y, Li J, Kang J, Xu X, Wang B, Lou S, Li J, Yang Y, Zhang Y, Zheng Y, Weng J, Liang Y, Ma W
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Feb;13(8):e04665

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Mouse oocytes sequester aggregated proteins in degradative super-organelles.

Zaffagnini G, Cheng S, Salzer MC, Pernaute B, Duran JM, Irimia M, Schuh M, Böke E
Cell 2024 Feb 29;187(5):1109-1126.e21

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Podocalyxin-like protein promotes gastric cancer progression through interacting with RUN and FYVE domain containing 1 protein.

Zhi Q, Chen H, Liu F, Han Y, Wan D, Xu Z, Kuang Y, Zhou J
Cancer science 2019 Jan;110(1):118-134

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Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5.

Shi M, Chen B, Mahajan D, Boh BK, Zhou Y, Dutta B, Tie HC, Sze SK, Wu G, Lu L
Nature communications 2018 Nov 26;9(1):4987

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COMMD1 is linked to the WASH complex and regulates endosomal trafficking of the copper transporter ATP7A.

Phillips-Krawczak CA, Singla A, Starokadomskyy P, Deng Z, Osborne DG, Li H, Dick CJ, Gomez TS, Koenecke M, Zhang JS, Dai H, Sifuentes-Dominguez LF, Geng LN, Kaufmann SH, Hein MY, Wallis M, McGaughran J, Gecz J, Sluis Bv, Billadeau DD, Burstein E
Molecular biology of the cell 2015 Jan 1;26(1):91-103

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Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks.

Gomez TS, Gorman JA, de Narvajas AA, Koenig AO, Billadeau DD
Molecular biology of the cell 2012 Aug;23(16):3215-28

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 13498-1-AP(RUFY1 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using RUFY1 antibody 13498-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP result of anti-RUFY1(13498-1-AP for IP and Detection).

Sample type

cell line