MA5-50060

antibody from Invitrogen Antibodies

Targeting: MAS1L dJ994E9.2, MAS-L, MRG

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-50060

Provider

Invitrogen Antibodies

Product name

MAS1L Recombinant Rabbit Monoclonal Antibody (15B8)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

15B8

Vial size

100 µL

Concentration

0.4 mg/mL

Storage

-20°C or -80°C if preferred

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of HeLa cell with MAS1L monoclonal antibody (Product # MA5-50060) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 595-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of MCF-7 cell with MAS1L monoclonal antibody (Product # MA5-50060) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 595-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis overlay peak curve showing Hela cells stained with (Product # MA5-50060) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at (1x10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.