MA5-25662

antibody from Invitrogen Antibodies

Targeting: IFI35 IFP35

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-25662

Provider

Invitrogen Antibodies

Product name

IFI35 Monoclonal Antibody (OTI1C9)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI1C9

Vial size

100 µL

Concentration

0.4 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Human Lentiviral Gene Therapy Restores the Cellular Phenotype of Autosomal Recessive Complete IFN-γR1 Deficiency.

Hahn K, Pollmann L, Nowak J, Nguyen AHH, Haake K, Neehus AL, Waqas SFH, Pessler F, Baumann U, Hetzel M, Casanova JL, Schulz A, Bustamante J, Ackermann M, Lachmann N
Molecular therapy. Methods & clinical development 2020 Jun 12;17:785-795

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of IFI35 in COS7 cells. Cells were transfected with a plasmid overexpressing IFI35 and probed with a IFI35 monoclonal antibody (Product # MA5-25662).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Evaluation of IFN-gammaR1 Expression and Functionality in Lv.SFFV.IFNgammaR1.iGFP Transduced IFN-gammaR1 -/- HeLa Cells (A) Representative histograms depicting IFN-gammaR1 (CD119) expression on WT (orange), IFN-gammaR1 -/- (knockout [KO], black), and Lv.SFFV.GFP (green) or Lv.SFFV.IFNgammaR1.iGFP (blue) transduced KO HeLa cells (unstained control cells are depicted in gray). (B) Quantification of median fluorescence intensities (MFIs) of IFN-gammaR1 (CD119) expression in WT, KO, and transduced HeLa cells (n = 3-4; mean +- SD, statistical analysis was performed using one-way-ANOVA with Tukey's post hoc testing. All shown values are compared to KO cells, *p