PA5-43777

antibody from Invitrogen Antibodies

Targeting: VAT1 FLJ20230, VATI

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-43777

Provider

Invitrogen Antibodies

Product name

VAT1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: PPAPGPGQLT LRLRACGLNF ADLMARQGLY DRLPPLPVTP GMEGAGVVIA Sequence homology: Cow: 93%; Dog: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 93%; Rat: 100%; Zebrafish: 86%

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

Chemical Proteomics Reveals Off-Targets of the Anandamide Reuptake Inhibitor WOBE437.

Gagestein B, Stevens AF, Fazio D, Florea BI, van der Wel T, Bakker AT, Fezza F, Dulk HD, Overkleeft HS, Maccarrone M, van der Stelt M
ACS chemical biology 2022 May 20;17(5):1174-1183

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Partial SCCPDH and VAT1 knockouts were generated by CRISPR-Cas9. (A) SCCPDH and (C) VAT1 KO Neuro-2a lines were generated and checked by gel-based AfBPP using 0.1 muM pac-WOBE (3) for residual expression. VAT1 protein was tested by VAT1 Western blot. Coomassie served as a protein loading control. Lipid levels were tested by lipidomics on (B) SCCPDH KO and (D) VAT1 KO cells and compared to WT cells. Further characterization and the complete list of ratios are depicted in Figures S6 and S7 . (E) Neuro-2a cells were treated with 10 muM WOBE437 or vehicle for 30 min and harvested to be analyzed by MS-based lipidomics. Lipid levels are displayed as ratio against the same type of cells treated with vehicle. Data represent means +- SEM ( n = 4). One-way ANOVA with Dunnett's multiple comparisons correction: not significant when compared to WT.