Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00050619-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00050619-M01, RRID:AB_2091558
- Product name
- Mouse Monoclonal DEF6 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. DEF6 - differentially expressed in FDCP 6 homolog (mouse)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 50 ug
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references DEF6 expression in ovarian carcinoma correlates with poor patient survival.
Def-6, a novel regulator of small GTPases in podocytes, acts downstream of atypical protein kinase C (aPKC) λ/ι.
Liew PL, Fang CY, Lee YC, Lee YC, Chen CL, Chu JS
Diagnostic pathology 2016 Aug 3;11(1):68
Diagnostic pathology 2016 Aug 3;11(1):68
Def-6, a novel regulator of small GTPases in podocytes, acts downstream of atypical protein kinase C (aPKC) λ/ι.
Worthmann K, Leitges M, Teng B, Sestu M, Tossidou I, Samson T, Haller H, Huber TB, Schiffer M
The American journal of pathology 2013 Dec;183(6):1945-1959
The American journal of pathology 2013 Dec;183(6):1945-1959
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: DEF6 Antibody (1F2) [H00050619-M01] - Detection limit for recombinant GST tagged DEF6 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: DEF6 Antibody (1F2) [H00050619-M01] - Analysis of DEF6 transfected lysate using anti-DEF6 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DEF6 monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DEF6 Antibody (1F2) [H00050619-M01] - Analysis of monoclonal antibody to DEF6 on formalin-fixed paraffin-embedded human lymph node Antibody concentration 3 ug/ml.