11369-1-AP

antibody from Invitrogen Antibodies

Targeting: DEF6 IBP, SLAT, SWAP70L

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11369-1-AP

Provider

Invitrogen Antibodies

Product name

DEF6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MALRKELLK SIWYAFTALD VEKSGKVSKS QLKVLSHNLY TVLHIPHDPV ALEEHFRDDD DGPVSSQGYM PYLNKYILDK VEEGAFVKEH FDELCWTLTA KKNYRADSNG NSMLSNQDAF RLWCLFNFLS EDKYPLIMVP DEVEYLLKKV LSSMSLEVSL GELEELLAQE AQVAQTTGGL SVWQFLELFN SGRCLRGVGR DTLSMAIHEV YQELIQDVLK QGYLWKRGHL RRNWAERWFQ LQPSCLCYFG SEECKEKRGI IPLDAHCCVE VLPDRDGKRC MFCVKTATRT YEMSASDTRQ RQEWTAAIQM AIRLQAEGKT SLHKDLKQKR REQREQR (1-336 aa encoded by BC017504)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.2 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of DEF6 antibody (11369-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DEF6 transfected Jurkat cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 11369-1-AP ( DEF6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 11369-1-AP (DEF6 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 11369-1-AP (DEF6 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse spleen tissue slide using 11369-1-AP ( DEF6 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse spleen tissue slide using 11369-1-AP ( DEF6 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-DEF6 (IP:11369-1-AP, 4ug; Detection:11369-1-AP 1:600) with Jurkat cells lysate 2400ug.