RQ5580

antibody from NSJ Bioreagents

Targeting: GRM5 GPRC1E, mGlu5, MGLUR5, PPP1R86

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5580

Provider

NSJ Bioreagents

Product name

GRM5 Antibody / Glutamate Metabotropic Receptor 5

Antibody type

Polyclonal

Description

This highly specific GRM5 antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the GRM5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat brain and 2) mouse brain lysate with GRM5 antibody. Expected molecular weight: routinely observed at 125-150 kDa (monomer) and 250+ kDa (dimer).

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human U937 cells with GRM5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GRM5 antibody.