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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF562 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat MuSK Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat MuSK in direct ELISAs and Western blots.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Characterization of pathogenic monoclonal autoantibodies derived from muscle-specific kinase myasthenia gravis patients.
DOK7 gene therapy enhances motor activity and life span in ALS model mice.
IgG-specific cell-based assay detects potentially pathogenic MuSK-Abs in seronegative MG.
Laminin is instructive and calmodulin dependent kinase II is non-permissive for the formation of complex aggregates of acetylcholine receptors on myotubes in culture.
MuSK myasthenia gravis IgG4 disrupts the interaction of LRP4 with MuSK but both IgG4 and IgG1-3 can disperse preformed agrin-independent AChR clusters.
Immunocapture and identification of cell membrane protein antigenic targets of serum autoantibodies.
Mutations causing DOK7 congenital myasthenia ablate functional motifs in Dok-7.
MuSK expressed in the brain mediates cholinergic responses, synaptic plasticity, and memory formation.
14-3-3 gamma associates with muscle specific kinase and regulates synaptic gene transcription at vertebrate neuromuscular synapse.
Postsynaptic requirement for Abl kinases in assembly of the neuromuscular junction.
Takata K, Stathopoulos P, Cao M, Mané-Damas M, Fichtner ML, Benotti ES, Jacobson L, Waters P, Irani SR, Martinez-Martinez P, Beeson D, Losen M, Vincent A, Nowak RJ, O'Connor KC
JCI insight 2019 Jun 20;4(12)
JCI insight 2019 Jun 20;4(12)
DOK7 gene therapy enhances motor activity and life span in ALS model mice.
Miyoshi S, Tezuka T, Arimura S, Tomono T, Okada T, Yamanashi Y
EMBO molecular medicine 2017 Jul;9(7):880-889
EMBO molecular medicine 2017 Jul;9(7):880-889
IgG-specific cell-based assay detects potentially pathogenic MuSK-Abs in seronegative MG.
Huda S, Waters P, Woodhall M, Leite MI, Jacobson L, De Rosa A, Maestri M, Ricciardi R, Heckmann JM, Maniaol A, Evoli A, Cossins J, Hilton-Jones D, Vincent A
Neurology(R) neuroimmunology & neuroinflammation 2017 Jul;4(4):e357
Neurology(R) neuroimmunology & neuroinflammation 2017 Jul;4(4):e357
Laminin is instructive and calmodulin dependent kinase II is non-permissive for the formation of complex aggregates of acetylcholine receptors on myotubes in culture.
Vezina-Audette R, Tremblay M, Carbonetto S
Matrix biology : journal of the International Society for Matrix Biology 2017 Jan;57-58:106-123
Matrix biology : journal of the International Society for Matrix Biology 2017 Jan;57-58:106-123
MuSK myasthenia gravis IgG4 disrupts the interaction of LRP4 with MuSK but both IgG4 and IgG1-3 can disperse preformed agrin-independent AChR clusters.
Koneczny I, Cossins J, Waters P, Beeson D, Vincent A
PloS one 2013;8(11):e80695
PloS one 2013;8(11):e80695
Immunocapture and identification of cell membrane protein antigenic targets of serum autoantibodies.
Littleton E, Dreger M, Palace J, Vincent A
Molecular & cellular proteomics : MCP 2009 Jul;8(7):1688-96
Molecular & cellular proteomics : MCP 2009 Jul;8(7):1688-96
Mutations causing DOK7 congenital myasthenia ablate functional motifs in Dok-7.
Hamuro J, Higuchi O, Okada K, Ueno M, Iemura S, Natsume T, Spearman H, Beeson D, Yamanashi Y
The Journal of biological chemistry 2008 Feb 29;283(9):5518-24
The Journal of biological chemistry 2008 Feb 29;283(9):5518-24
MuSK expressed in the brain mediates cholinergic responses, synaptic plasticity, and memory formation.
Garcia-Osta A, Tsokas P, Pollonini G, Landau EM, Blitzer R, Alberini CM
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Jul 26;26(30):7919-32
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Jul 26;26(30):7919-32
14-3-3 gamma associates with muscle specific kinase and regulates synaptic gene transcription at vertebrate neuromuscular synapse.
Strochlic L, Cartaud A, Mejat A, Grailhe R, Schaeffer L, Changeux JP, Cartaud J
Proceedings of the National Academy of Sciences of the United States of America 2004 Dec 28;101(52):18189-94
Proceedings of the National Academy of Sciences of the United States of America 2004 Dec 28;101(52):18189-94
Postsynaptic requirement for Abl kinases in assembly of the neuromuscular junction.
Finn AJ, Feng G, Pendergast AM
Nature neuroscience 2003 Jul;6(7):717-23
Nature neuroscience 2003 Jul;6(7):717-23
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Acetylcholine Receptor Clustering Mediated by MuSK and Neutralization by Rat MuSK Antibody. Recombinant Rat Agrin (Catalog # 550-AG) induces MuSK-dependent acetylcholine receptor clustering on myotubes differentiated from the C2C12 mouse myoblast cell line in a dose-dependent manner (orange line). Acetylcholine Receptor Clustering elicited by Recombinant Rat Agrin (16 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat MuSK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF562). The ND50 is typically 0.5-2 µg/mL.
