MA5-24179

antibody from Invitrogen Antibodies

Targeting: TSPAN8 CO-029, TM4SF3

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24179

Provider

Invitrogen Antibodies

Product name

TSPAN8 Monoclonal Antibody (458811)

Antibody type

Monoclonal

Antigen

Other

Description

Reconstitute at 0.5 mg/mL in sterile PBS.

Reactivity

Human

Host

Rat

Isotype

IgG

Antibody clone number

458811

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

PyMINEr Finds Gene and Autocrine-Paracrine Networks from Human Islet scRNA-Seq.

Tyler SR, Rotti PG, Sun X, Yi Y, Xie W, Winter MC, Flamme-Wiese MJ, Tucker BA, Mullins RF, Norris AW, Engelhardt JF
Cell reports 2019 Feb 12;26(7):1951-1964.e8

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of TSPAN8 in immersion fixed HT‚29 human colon adenocarcinoma cell line. Samples were incubated in TSPAN8 monoclonal antibody (Product # MA5-24179) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red) and counterstained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of TSPAN8 in immersion fixed HT‚29 human colon adenocarcinoma cell line. Samples were incubated in TSPAN8 monoclonal antibody (Product # MA5-24179) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red) and counterstained with DAPI (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HT29 human colon adenocarcinoma cell line was stained with Rat Anti-human TSPAN8 Monoclonal (Product # MA5-24179, filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F (ab')2 Secondary Antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of TSPAN8 in HT‚29 human colon adenocarcinoma cell line. Samples were incubated in TSPAN8 monoclonal antibody (Product # MA5-24179) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab)2 Secondary Antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of TSPAN8 in HT‚29 human colon adenocarcinoma cell line. Samples were incubated in TSPAN8 monoclonal antibody (Product # MA5-24179) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab)2 Secondary Antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5. Cell Type-Specific Enrichment of T2D-Associated Genes (A) A subset of the larger networks shown in Figure 2E focused on T2D-associated genes. The nature of the associations that were previously published is indicated by color ( Morris et al., 2012 ). Gene associations newly discovered through the cited meta-analysis are denoted as ""newly associated locus."" (B-I) The Z score enrichment of T2D-associated genes by cell type (from our dataset) are displayed over the two largest connected components of the T2D gene subset of the transcriptional network built by PyMINEr. As indicated, panels correspond to beta (B), epsilon (C), pancreatic polypeptide cells (D), ductal (E), stromal (F), delta (G), acinar (H), and alpha cells (I). Highly enriched genes are shown in red, whereas genes whose expression is low in the given cell type are shown in blue. If a gene passed the threshold for significant enrichment for the given cell type, then it is highlighted with a cyan ring. Table S2A lists cell type annotations for all T2D-associated genes, including those not shown here. (J) Immunofluorescence staining of a human pancreas section for glucagon (GCG; alpha cells, red), BSCL2 (green), and insulin (INS; beta cells, white) and counterstaining with Hoechst dye (nuclei, blue) (n = 5). Although many alpha cells were positive for BSCL2, we also observed expression in other islet cells (examples noted with arrows). (K) Representative immunofluorescence staining of a human pancreas sec