HPA014518

antibody from Atlas Antibodies

Targeting: P2RY12 HORK3, P2Y12, SP1999

Provider product page for HPA014518

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [57]
Comments [0]
Validations
Immunohistochemistry [1]

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Product number: HPA014518 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA014518, RRID:AB_2669027
Product name: Anti-P2RY12
Antibody type: Polyclonal
Description: Polyclonal Antibody against Human P2RY12, Gene description: purinergic receptor P2Y, G-protein coupled, 12, Alternative Gene Names: HORK3, P2Y12, SP1999, Validated applications: IHC, Uniprot ID: Q9H244, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 µl
Concentration: 0.2 mg/ml
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Handling: The antibody solution should be gently mixed before use.

P2RY12 protein structure - HPA014518 shown in red.

Submitted references Pro-resolving lipid mediator reduces amyloid-β42–induced gene expression in human monocyte–derived microglia

Wang Y, Zhang X, Biverstål H, Bazan N, Tan S, Li N, Ohshima M, Schultzberg M, Li X
Neural Regeneration Research 2025;20(3):873-886

Age-associated microglial transcriptome leads to diminished immunogenicity and dysregulation of MCT4 and P2RY12/P2RY13 related functions

Škandík M, Friess L, Vázquez-Cabrera G, Keane L, Grabert K, Cruz De los Santos M, Posada-Pérez M, Baleviciute A, Cheray M, Joseph B
Cell Death Discovery 2025;11(1)

Retinal Organoid Microenvironment Enhanced Bioactivities of Microglia-Like Cells Derived From HiPSCs

Gao M, Wang T, Lin X, Tang C, Li M, Bai Z, Liu Z, Chen L, Kong Q, Pan S, Zeng S, Guo Y, Cai J, Huang X, Zhang J
Investigative Ophthalmology & Visual Science 2024;65(12):19

Human Microglia–Like Cells Differentiated from Monocytes with GM-CSF and IL-34 Show Phagocytosis of α-Synuclein Aggregates and C/EBPβ-Dependent Proinflammatory Activation

Llaves-López A, Micoli E, Belmonte-Mateos C, Aguilar G, Alba C, Marsal A, Pulido-Salgado M, Rabaneda-Lombarte N, Solà C, Serratosa J, Vidal-Taboada J, Saura J
Molecular Neurobiology 2024;62(1):756-772

Xenografted human microglia display diverse transcriptomic states in response to Alzheimer’s disease-related amyloid-β pathology

Mancuso R, Fattorelli N, Martinez-Muriana A, Davis E, Wolfs L, Van Den Daele J, Geric I, Premereur J, Polanco P, Bijnens B, Preman P, Serneels L, Poovathingal S, Balusu S, Verfaillie C, Fiers M, De Strooper B
Nature Neuroscience 2024;27(5):886-900

Deterioration of neuroimmune homeostasis in Alzheimer’s Disease patients who survive a COVID-19 infection

Villareal J, Bathe T, Hery G, Phillips J, Tsering W, Prokop S
Journal of Neuroinflammation 2024;21(1)

Sodium oligomannate alters gut microbiota, reduces cerebral amyloidosis and reactive microglia in a sex-specific manner

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Molecular Neurodegeneration 2024;19(1)

Human VCP mutant ALS/FTD microglia display immune and lysosomal phenotypes independently of GPNMB

Clarke B, Ziff O, Tyzack G, Petrić Howe M, Wang Y, Klein P, Smith C, Hall C, Helmy A, Howell M, Kelly G, Patani R
Molecular Neurodegeneration 2024;19(1)

Brain organoids engineered to give rise to glia and neural networks after 90 days in culture exhibit human-specific proteoforms.

Wenzel TJ, Mousseau DD
Frontiers in cellular neuroscience 2024;18:1383688

Reliable detection of RNA in hippocampus sections of mice by FISH up to a post-mortem delay of 24 h

Seiffer S, Brendler J, Schulz A, Ricken A
Histochemistry and Cell Biology 2024;161(6):539-547

Interaction between subventricular zone microglia and neural stem cells impacts the neurogenic response in a mouse model of cortical ischemic stroke

Nath S, Martínez Santamaría J, Chu Y, Choi J, Conforti P, Lin J, Sankowski R, Amann L, Galanis C, Wu K, Deshpande S, Vlachos A, Prinz M, Lee J, Schachtrup C
Nature Communications 2024;15(1)

Ready-to-use iPSC-derived microglia progenitors for the treatment of CNS disease in mouse models of neuropathic mucopolysaccharidoses

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Nature Communications 2024;15(1)

Disease and brain region specific immune response profiles in neurodegenerative diseases with pure and mixed protein pathologies

Bathe T, Hery G, Villareal J, Phillips J, Cohen E, Sharma R, Tsering W, Prokop S
Acta Neuropathologica Communications 2024;12(1)

Human-induced pluripotent stem cell-derived microglia integrate into mouse retina and recapitulate features of endogenous microglia

Ma W, Zhao L, Xu B, Fariss R, Redmond T, Zou J, Wong W, Li W
eLife 2024;12

Polygenic risk for alcohol use disorder affects cellular responses to ethanol exposure in a human microglial cell model

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Science Advances 2024;10(45)

Hypoxia coordinates the spatial landscape of myeloid cells within glioblastoma to affect survival

Haley M, Bere L, Minshull J, Georgaka S, Garcia-Martin N, Howell G, Coope D, Roncaroli F, King A, Wedge D, Allan S, Pathmanaban O, Brough D, Couper K
Science Advances 2024;10(20)

Dual targeting macrophages and microglia is a therapeutic vulnerability in models of PTEN-deficient glioblastoma.

Liu Y, Wu J, Najem H, Lin Y, Pang L, Khan F, Zhou F, Ali H, Heimberger AB, Chen P
The Journal of clinical investigation 2024 Oct 1;134(22)

Sustained type I interferon signaling after human immunodeficiency virus type 1 infection of human iPSC derived microglia and cerebral organoids.

Boreland AJ, Stillitano AC, Lin HC, Abbo Y, Hart RP, Jiang P, Pang ZP, Rabson AB
iScience 2024 May 17;27(5):109628

Inflammation-induced TRPV4 channels exacerbate blood-brain barrier dysfunction in multiple sclerosis.

Hansen CE, Kamermans A, Mol K, Berve K, Rodriguez-Mogeda C, Fung WK, van Het Hof B, Fontijn RD, van der Pol SMA, Michalick L, Kuebler WM, Kenkhuis B, van Roon-Mom W, Liedtke W, Engelhardt B, Kooij G, Witte ME, de Vries HE
Journal of neuroinflammation 2024 Mar 23;21(1):72

Human microglia maturation is underpinned by specific gene regulatory networks

Han C, Li R, Hansen E, Trescott S, Fixsen B, Nguyen C, Mora C, Spann N, Bennett H, Poirion O, Buchanan J, Warden A, Xia B, Schlachetzki J, Pasillas M, Preissl S, Wang A, O’Connor C, Shriram S, Kim R, Schafer D, Ramirez G, Challacombe J, Anavim S, Johnson A, Gupta M, Glass I, Levy M, Haim S, Gonda D, Laurent L, Hughes J, Page D, Blurton-Jones M, Glass C, Coufal N
Immunity 2023;56(9):2152-2171.e13

Human astrocytes and microglia show augmented ingestion of synapses in Alzheimer’s disease via MFG-E8

Tzioras M, Daniels M, Davies C, Baxter P, King D, McKay S, Varga B, Popovic K, Hernandez M, Stevenson A, Barrington J, Drinkwater E, Borella J, Holloway R, Tulloch J, Moss J, Latta C, Kandasamy J, Sokol D, Smith C, Miron V, Káradóttir R, Hardingham G, Henstridge C, Brennan P, McColl B, Spires-Jones T
Cell Reports Medicine 2023;4(9):101175

Engineering an inhibitor-resistant human CSF1R variant for microglia replacement

Chadarevian J, Lombroso S, Peet G, Hasselmann J, Tu C, Marzan D, Capocchi J, Purnell F, Nemec K, Lahian A, Escobar A, England W, Chaluvadi S, O’Brien C, Yaqoob F, Aisenberg W, Porras-Paniagua M, Bennett M, Davtyan H, Spitale R, Blurton-Jones M, Bennett F
Journal of Experimental Medicine 2023;220(3)

Translational profiling identifies sex-specific metabolic and epigenetic reprogramming of cortical microglia/macrophages in APPPS1-21 mice with an antibiotic-perturbed-microbiome

Shaik S, Cao Y, Gogola J, Dodiya H, Zhang X, Boutej H, Han W, Kriz J, Sisodia S
Molecular Neurodegeneration 2023;18(1)

Moderate intrinsic phenotypic alterations in C9orf72 ALS/FTD iPSC-microglia despite the presence of C9orf72 pathological features

Lorenzini I, Alsop E, Levy J, Gittings L, Lall D, Rabichow B, Moore S, Pevey R, Bustos L, Burciu C, Bhatia D, Singer M, Saul J, McQuade A, Tzioras M, Mota T, Logemann A, Rose J, Almeida S, Gao F, Marks M, Donnelly C, Hutchins E, Hung S, Ichida J, Bowser R, Spires-Jones T, Blurton-Jones M, Gendron T, Baloh R, Van Keuren-Jensen K, Sattler R
Frontiers in Cellular Neuroscience 2023;17

Defects in lysosomal function and lipid metabolism in human microglia harboring a TREM2 loss of function mutation

Filipello F, You S, Mirfakhar F, Mahali S, Bollman B, Acquarone M, Korvatska O, Marsh J, Sivaraman A, Martinez R, Cantoni C, De Feo L, Ghezzi L, Minaya M, Renganathan A, Cashikar A, Satoh J, Beatty W, Iyer A, Cella M, Raskind W, Piccio L, Karch C
Acta Neuropathologica 2023;145(6):749-772

CSF1R inhibitors induce a sex-specific resilient microglial phenotype and functional rescue in a tauopathy mouse model

Johnson N, Yuan P, Castillo E, Lopez T, Yue W, Bond A, Rivera B, Sullivan M, Hirouchi M, Giles K, Aoyagi A, Condello C
Nature Communications 2023;14(1)

Human iPSC-derived microglia carrying the LRRK2-G2019S mutation show a Parkinson’s disease related transcriptional profile and function

Ohtonen S, Giudice L, Jäntti H, Fazaludeen M, Shakirzyanova A, Gómez-Budia M, Välimäki N, Niskanen J, Korvenlaita N, Fagerlund I, Koistinaho J, Amiry-Moghaddam M, Savchenko E, Roybon L, Lehtonen Š, Korhonen P, Malm T
Scientific Reports 2023;13(1)

The localization of molecularly distinct microglia populations to Alzheimer's disease pathologies using QUIVER

Shahidehpour R, Nelson A, Sanders L, Embry C, Nelson P, Bachstetter A
Acta Neuropathologica Communications 2023;11(1)

Min A, Javidfar B, Missall R, Doanman D, Durens M, Vil S, Masih Z, Graziani M, Mordelt A, Marro S, de Witte L, Chen B, Swartz T, Akbarian S
2023

A single-cell atlas of the normal and malformed human brain vasculature

Winkler E, Kim C, Ross J, Garcia J, Gil E, Oh I, Chen L, Wu D, Catapano J, Raygor K, Narsinh K, Kim H, Weinsheimer S, Cooke D, Walcott B, Lawton M, Gupta N, Zlokovic B, Chang E, Abla A, Lim D, Nowakowski T
Science 2022;375(6584)

Assessing human iPSC-derived microglia identity and function by immunostaining, phagocytosis, calcium activity, and inflammation assay

Hübschmann V, Korkut-Demirbaş M, Siegert S
STAR Protocols 2022;3(4):101866

A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation

Bartalska K, Hübschmann V, Korkut-Demirbaş M, Cubero R, Venturino A, Rössler K, Czech T, Siegert S
iScience 2022;25(7):104580

Cholesterol and matrisome pathways dysregulated in astrocytes and microglia

TCW J, Qian L, Pipalia N, Chao M, Liang S, Shi Y, Jain B, Bertelsen S, Kapoor M, Marcora E, Sikora E, Andrews E, Martini A, Karch C, Head E, Holtzman D, Zhang B, Wang M, Maxfield F, Poon W, Goate A
Cell 2022;185(13):2213-2233.e25

Neurodegenerative phagocytes mediate synaptic stripping in Neuro-HIV

Di Liberto G, Egervari K, Kreutzfeldt M, Schürch C, Hewer E, Wagner I, Du Pasquier R, Merkler D
Brain 2022;145(8):2730-2741

Advances in Visualizing Microglial Cells in Human Central Nervous System Tissue

Uff C, Patel K, Yeung C, Yip P
Biomolecules 2022;12(5):603

Human neural cell type‐specific extracellular vesicle proteome defines disease‐related molecules associated with activated astrocytes in Alzheimer's disease brain

You Y, Muraoka S, Jedrychowski M, Hu J, McQuade A, Young‐Pearse T, Aslebagh R, Shaffer S, Gygi S, Blurton‐Jones M, Poon W, Ikezu T
Journal of Extracellular Vesicles 2022;11(1)

Expression of HIV-1 Intron-Containing RNA in Microglia Induces Inflammatory Responses

Akiyama H, Jalloh S, Park S, Lei M, Mostoslavsky G, Gummuluru S, Simon V
Journal of Virology 2021;95(5)

Microglia‐specific ApoE knock‐out does not alter Alzheimer's disease plaque pathogenesis or gene expression

Henningfield C, Arreola M, Soni N, Spangenberg E, Green K
Glia 2021;70(2):287-302

Microglia enable mature perineuronal nets disassembly upon anesthetic ketamine exposure or 60-Hz light entrainment in the healthy brain

Venturino A, Schulz R, De Jesús-Cortés H, Maes M, Nagy B, Reilly-Andújar F, Colombo G, Cubero R, Schoot Uiterkamp F, Bear M, Siegert S
Cell Reports 2021;36(1):109313

Human microglia states are conserved across experimental models and regulate neural stem cell responses in chimeric organoids

Popova G, Soliman S, Kim C, Keefe M, Hennick K, Jain S, Li T, Tejera D, Shin D, Chhun B, McGinnis C, Speir M, Gartner Z, Mehta S, Haeussler M, Hengen K, Ransohoff R, Piao X, Nowakowski T
Cell Stem Cell 2021;28(12):2153-2166.e6

Integration of Alzheimer’s disease genetics and myeloid genomics identifies disease risk regulatory elements and genes

Novikova G, Kapoor M, TCW J, Abud E, Efthymiou A, Chen S, Cheng H, Fullard J, Bendl J, Liu Y, Roussos P, Björkegren J, Liu Y, Poon W, Hao K, Marcora E, Goate A
Nature Communications 2021;12(1)

Improved modeling of human AD with an automated culturing platform for iPSC neurons, astrocytes and microglia

Bassil R, Shields K, Granger K, Zein I, Ng S, Chih B
Nature Communications 2021;12(1)

Reporter cell assay for human CD33 validated by specific antibodies and human iPSC-derived microglia

Wißfeld J, Mathews M, Mossad O, Picardi P, Cinti A, Redaelli L, Pradier L, Brüstle O, Neumann H
Scientific Reports 2021;11(1)

Subventricular zone/white matter microglia reconstitute the empty adult microglial niche in a dynamic wave

Hohsfield L, Najafi A, Ghorbanian Y, Soni N, Crapser J, Figueroa Velez D, Jiang S, Royer S, Kim S, Henningfield C, Anderson A, Gandhi S, Mortazavi A, Inlay M, Green K
eLife 2021;10

Functional analysis of CX3CR1 in human induced pluripotent stem (iPS) cell‐derived microglia‐like cells

Murai N, Mitalipova M, Jaenisch R
European Journal of Neuroscience 2020;52(7):3667-3678

Microglia promote glioblastoma via mTOR‐mediated immunosuppression of the tumour microenvironment

Dumas A, Pomella N, Rosser G, Guglielmi L, Vinel C, Millner T, Rees J, Aley N, Sheer D, Wei J, Marisetty A, Heimberger A, Bowman R, Brandner S, Joyce J, Marino S
The EMBO Journal 2020;39(15)

Patterns of Expression of Purinergic Receptor P2RY12, a Putative Marker for Non-Activated Microglia, in Aged and Alzheimer’s Disease Brains

Walker D, Tang T, Mendsaikhan A, Tooyama I, Serrano G, Sue L, Beach T, Lue L
International Journal of Molecular Sciences 2020;21(2):678

Comparative analysis of human microglial models for studies of HIV replication and pathogenesis

Rai M, Hammonds J, Pujato M, Mayhew C, Roskin K, Spearman P
Retrovirology 2020;17(1)

Effects of long-term and brain-wide colonization of peripheral bone marrow-derived myeloid cells in the CNS

Hohsfield L, Najafi A, Ghorbanian Y, Soni N, Hingco E, Kim S, Jue A, Swarup V, Inlay M, Green K
Journal of Neuroinflammation 2020;17(1)

Quantitative immunohistochemical analysis of myeloid cell marker expression in human cortex captures microglia heterogeneity with anatomical context

Swanson M, Murray H, Ryan B, Faull R, Dragunow M, Curtis M
Scientific Reports 2020;10(1)

Absence of TGFβ signaling in retinal microglia induces retinal degeneration and exacerbates choroidal neovascularization

Ma W, Silverman S, Zhao L, Villasmil R, Campos M, Amaral J, Wong W
eLife 2019;8

Perivascular macrophages in the neonatal macaque brain undergo massive necroptosis after simian immunodeficiency virus infection

Bohannon D, Wang Y, Reinhart C, Hattler J, Luo J, Okhravi H, Zhang J, Li Q, Kuroda M, Kim J, Kim W
Brain Pathology 2019;30(3):603-613

Stem-cell-derived human microglia transplanted in mouse brain to study human disease

Mancuso R, Van Den Daele J, Fattorelli N, Wolfs L, Balusu S, Burton O, Liston A, Sierksma A, Fourne Y, Poovathingal S, Arranz-Mendiguren A, Sala Frigerio C, Claes C, Serneels L, Theys T, Perry V, Verfaillie C, Fiers M, De Strooper B
Nature Neuroscience 2019;22(12):2111-2116

Development and validation of a simplified method to generate human microglia from pluripotent stem cells

McQuade A, Coburn M, Tu C, Hasselmann J, Davtyan H, Blurton-Jones M
Molecular Neurodegeneration 2018;13(1)

iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases

Abud E, Ramirez R, Martinez E, Healy L, Nguyen C, Newman S, Yeromin A, Scarfone V, Marsh S, Fimbres C, Caraway C, Fote G, Madany A, Agrawal A, Kayed R, Gylys K, Cahalan M, Cummings B, Antel J, Mortazavi A, Carson M, Poon W, Blurton-Jones M
Neuron 2017;94(2):278-293.e9

Post-hypoxia Invasion of the fetal brain by multidrug resistant Staphylococcus

Zarate M, Rodriguez M, Chang E, Russell J, Arndt T, Richards E, Ocasio B, Aranda E, Gordon E, Yu K, Neu J, Keller-Wood M, Triplett E, Wood C
Scientific Reports 2017;7(1)

Ghosts in the shell: identification of microglia in the human central nervous system by P2Y12 receptor

Mildner A
Neural Regeneration Research 2017;12(4):570

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human cerebral cortex and liver tissues using HPA014518 antibody. Corresponding P2RY12 RNA-seq data are presented for the same tissues.
Sample type: Human
Protocol: Protocol
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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