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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- A300-672A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAML1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MAML1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, A-549, MCF-7, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAML1 antibody (Product # A300-672A; lot A300-672A-2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MAML1 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 µg for Input; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAML1 antibody BL1238 (Cat. No. A300-672A) used for WB at 1 µg/ml and for IP at 3 µg/mg lysate. MAML1 was also immunoprecipitated by rabbit anti-MAML1 antibodies BL1237 (Cat. No. A300-671A) and BL1239 (Cat. No. A300-673A) using 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MAML1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MAML1 (Cat. No. A300-672A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MAML1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAML1 antibody (Product # A300-672A; lot 2) used for IP at 6 µg per reaction. MAML1 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-MAML1 antibody (Product # A300-673A). For blotting immunoprecipitated MAML1, Product # A300-672A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
