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antibody from Invitrogen Antibodies
Targeting: SRSF7
9G8, AAG3, HSSG1, RBM37, SFRS7, ZCCHC20, ZCRB2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-39482 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-SRSF7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Neuroepithelial cell competition triggers loss of cellular juvenescence.
The juvenility-associated long noncoding RNA Gm14230 maintains cellular juvenescence.
Jam FA, Morimune T, Tsukamura A, Tano A, Tanaka Y, Mori Y, Yamamoto T, Nishimura M, Tooyama I, Mori M
Scientific reports 2020 Oct 22;10(1):18044
Scientific reports 2020 Oct 22;10(1):18044
The juvenility-associated long noncoding RNA Gm14230 maintains cellular juvenescence.
Tano A, Kadota Y, Morimune T, Jam FA, Yukiue H, Bellier JP, Sokoda T, Maruo Y, Tooyama I, Mori M
Journal of cell science 2019 Apr 16;132(8)
Journal of cell science 2019 Apr 16;132(8)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SFRS7 in extracts from HT-29 cells using a SFRS7 polyclonal antibody (Product # PA5-39482).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Neuroepithelial cell competition triggers Srsf7 loss in RAS V12 cells via proteasome-mediated degradation. ( a ) Representative images of Srsf7 expression in non-competitive and competitive condition. Arrows indicate Srsf7 loss in cells. Scale bars = 10 um. ( b ) Frequency of Srsf7 positive cells in non-competitive and competitive condition. ( c ) Western blot analysis of Srsf7 expression in the non-competitive condition upon doxycycline induction. Gapdh was used as a loading control. ( d ) Ubiquitylation assay of Srsf7. Cells were transfected with HA-tagged ubiquitin and treated with MG132, 5 uM for 8 h before lysate collection. The ubiquitylation of Srsf7 was determined by IP-western. Note that samples were derived from the same experiment and blots were processed in parallel. Full length blots are presented in Supplementary Fig. S4 . ( e ) Representative images of Srsf7 expression in the absence and presence of proteasome inhibitor MG132 during competitive condition. Arrows indicate Srsf7 positive cells. Scale bars = 20 um. ( f ) Frequency of Srsf7 positive cells in the absence and presence of proteasome inhibitor MG132 during competitive condition. ( g ) Number of RAS V12 cells counted after 24 h in the competitive condition of normal cells with control TdTomato RAS V12 cells or HA-Srsf7 overexpressed RAS V12 cells. ( h ) Frequency of caspase-3 positive in control TdTomato RAS V12 cells or HA-Srsf7 overexpressed RAS V12 cells during competitive condition. Data ar
