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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [2]
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- Product number
- PA3-035 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ECM29 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA3-035 detects Ecm29 in human and mouse samples. PA3-035 has been used successfully in Western blot and immunofluorescence procedures. In Western blot analysis of HeLa cell extracts this antibody detects a ~205 kDa protein representing Ecm29. The PA3-035 immunogen is a synthetic peptide corresponding to the C-terminal residues R(1821) P E L Q E K A A L L K K T L E N L E(1839) of human Ecm29.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Characterization of the Brain 26S Proteasome and its Interacting Proteins.
Characterization of mammalian Ecm29, a 26 S proteasome-associated protein that localizes to the nucleus and membrane vesicles.
Tai HC, Besche H, Goldberg AL, Schuman EM
Frontiers in molecular neuroscience 2010;3
Frontiers in molecular neuroscience 2010;3
Characterization of mammalian Ecm29, a 26 S proteasome-associated protein that localizes to the nucleus and membrane vesicles.
Gorbea C, Goellner GM, Teter K, Holmes RK, Rechsteiner M
The Journal of biological chemistry 2004 Dec 24;279(52):54849-61
The Journal of biological chemistry 2004 Dec 24;279(52):54849-61
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ecm29 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ecm29 Rabbit Polyclonal Antibody (Product # PA3-035) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Ecm29 in HeLa cells using Product # PA3-035.
