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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB9984 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9984, RRID:AB_1672183
- Product name
- CAMK2G polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CAMK2G.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Synaptic vesicle-associated Ca2+/calmodulin-dependent protein kinase II is a binding protein for synapsin I.
Sequence analysis and DNA-protein interactions within the 5' flanking region of the Ca2+/calmodulin-dependent protein kinase II alpha-subunit gene.
Ca2+/calmodulin-dependent protein kinase II: identification of threonine-286 as the autophosphorylation site in the alpha subunit associated with the generation of Ca2+-independent activity.
Benfenati F, Valtorta F, Rubenstein JL, Gorelick FS, Greengard P, Czernik AJ
Nature 1992 Oct 1;359(6394):417-20
Nature 1992 Oct 1;359(6394):417-20
Sequence analysis and DNA-protein interactions within the 5' flanking region of the Ca2+/calmodulin-dependent protein kinase II alpha-subunit gene.
Sunyer T, Sahyoun N
Proceedings of the National Academy of Sciences of the United States of America 1990 Jan;87(1):278-82
Proceedings of the National Academy of Sciences of the United States of America 1990 Jan;87(1):278-82
Ca2+/calmodulin-dependent protein kinase II: identification of threonine-286 as the autophosphorylation site in the alpha subunit associated with the generation of Ca2+-independent activity.
Thiel G, Czernik AJ, Gorelick F, Nairn AC, Greengard P
Proceedings of the National Academy of Sciences of the United States of America 1988 Sep;85(17):6337-41
Proceedings of the National Academy of Sciences of the United States of America 1988 Sep;85(17):6337-41
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using CAMK2G polyclonal antibody (Cat # PAB9984) shows detectionof a band ~54 KDa corresponding to human CAMK2G (arrowhead lane 1).Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (Lane 2).Approximately 35 ug of a mouse brain tissue lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.CAMK2G was similarly detected on lysates from rat brain (not shown).After blocking the membrane was probed with the primary antibody diluted to 1 : 1,500 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademarkof LI-COR, Inc.
