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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- AF7280 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CaM Kinase II gamma Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CaM Kinase II gamma in direct ELISAs and Western blots
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q13555- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CaM Kinase II gamma by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CaM Kinase II gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7280) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CaM Kinase II gamma at approximately 55-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CaM Kinase II gamma in Human Brain. CaM Kinase II gamma was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Sheep Anti-Human CaM Kinase II gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7280) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CaM Kinase II gamma in Mouse Brain. CaM Kinase II gamma was detected in perfusion fixed frozen sections of mouse brain (brainstem) using Sheep Anti-Human CaM Kinase II gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7280) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
