MA5-17066

antibody from Invitrogen Antibodies

Targeting: DCX DBCN, DC, LISX, SCLH, XLIS

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-17066

Provider

Invitrogen Antibodies

Product name

Doublecortin Monoclonal Antibody (2G5)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-17066 targets DCX in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples. The MA5-17066 immunogen is purified recombinant fragment of human DCX (amino acids: 362-411) expressed in E. Coli. MA5-17066 detects DCX which has a predicted molecular weight of approximately 49.3kDa.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

2G5

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Neuroprotection of N-benzyl Eicosapentaenamide in Neonatal Mice Following Hypoxic-Ischemic Brain Injury.

Jiao M, Dong Q, Zhang Y, Lin M, Zhou W, Liu T, Yuan B, Yin H
Molecules (Basel, Switzerland) 2021 May 22;26(11)

---

A Combinatorial MAP Code Dictates Polarized Microtubule Transport.

Monroy BY, Tan TC, Oclaman JM, Han JS, Simó S, Niwa S, Nowakowski DW, McKenney RJ, Ori-McKenney KM
Developmental cell 2020 Apr 6;53(1):60-72.e4

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DCX using a DCX monoclonal antibody (Product # MA5-17066) against a human DCX recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DCX using a DCX monoclonal antibody (Product # MA5-17066) against a human DCX recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DCX using DCX monoclonal antibody (Product # MA5-17066) in mouse heart (1) lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HepG2 cells using DCX monoclonal antibody (Product # MA5-17066) (Green). Blue: DRAQ5 fluorescent DNA dye.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HepG2 cells using DCX monoclonal antibody (Product # MA5-17066) (Green). Blue: DRAQ5 fluorescent DNA dye.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HepG2 cells using DCX monoclonal antibody (Product # MA5-17066) (Green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded brain tissue tissues using DCX monoclonal antibody (Product # MA5-17066) followed with DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using DCX monoclonal antibody (Product # MA5-17066) followed with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of SK-N-SH cells using DCX monoclonal antibody (Product # MA5-17066) (green) and negative control (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 NB-EPA improves the neuronal survival and proliferation in neonatal mice after HI brain injury. ( A ) Representative photographs of Nissl staining in the cortex, hippocampus DG, CA1 and CA3 regions from every group of mice at 7 days post-HI (Scale bar, 75 mum). ( B ) Statistical analysis of the number of neurons is shown in ( A ). ( C ) Representative Western blot images of p-AKT and AKT from every group of mice at 7 days after HI. ( D ) Densitometric analysis of data is shown in ( C ). ( E ) Representative images of DCX (green) and Ki-67 (red) from every group of mice at 7 days after HI (left, Scale bar, 25 mum) in hippocampus DG regions; quantification of DCX and Ki-67 dual-labeled cells at 7 days post-HI (right). Data are mean +- SEM ( n = 8 per group). * p < 0.05 compared to vehicle.