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- Immunocytochemistry [3]
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- Product number
- MA5-17066 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Doublecortin Monoclonal Antibody (2G5)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17066 targets DCX in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples. The MA5-17066 immunogen is purified recombinant fragment of human DCX (amino acids: 362-411) expressed in E. Coli. MA5-17066 detects DCX which has a predicted molecular weight of approximately 49.3kDa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2G5
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Neuroprotection of N-benzyl Eicosapentaenamide in Neonatal Mice Following Hypoxic-Ischemic Brain Injury.
A Combinatorial MAP Code Dictates Polarized Microtubule Transport.
Jiao M, Dong Q, Zhang Y, Lin M, Zhou W, Liu T, Yuan B, Yin H
Molecules (Basel, Switzerland) 2021 May 22;26(11)
Molecules (Basel, Switzerland) 2021 May 22;26(11)
A Combinatorial MAP Code Dictates Polarized Microtubule Transport.
Monroy BY, Tan TC, Oclaman JM, Han JS, Simó S, Niwa S, Nowakowski DW, McKenney RJ, Ori-McKenney KM
Developmental cell 2020 Apr 6;53(1):60-72.e4
Developmental cell 2020 Apr 6;53(1):60-72.e4
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HepG2 cells using DCX monoclonal antibody (Product # MA5-17066) (Green). Blue: DRAQ5 fluorescent DNA dye.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HepG2 cells using DCX monoclonal antibody (Product # MA5-17066) (Green). Blue: DRAQ5 fluorescent DNA dye.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HepG2 cells using DCX monoclonal antibody (Product # MA5-17066) (Green). Blue: DRAQ5 fluorescent DNA dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of SK-N-SH cells using DCX monoclonal antibody (Product # MA5-17066) (green) and negative control (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 NB-EPA improves the neuronal survival and proliferation in neonatal mice after HI brain injury. ( A ) Representative photographs of Nissl staining in the cortex, hippocampus DG, CA1 and CA3 regions from every group of mice at 7 days post-HI (Scale bar, 75 mum). ( B ) Statistical analysis of the number of neurons is shown in ( A ). ( C ) Representative Western blot images of p-AKT and AKT from every group of mice at 7 days after HI. ( D ) Densitometric analysis of data is shown in ( C ). ( E ) Representative images of DCX (green) and Ki-67 (red) from every group of mice at 7 days after HI (left, Scale bar, 25 mum) in hippocampus DG regions; quantification of DCX and Ki-67 dual-labeled cells at 7 days post-HI (right). Data are mean +- SEM ( n = 8 per group). * p < 0.05 compared to vehicle.
